不同剂量链尿佐菌素诱导2型糖尿病大鼠模型的研究  

Study on Type 2 Diabetic Rat Models induced by Different Doses of Streptozotocin

作　　者：余震[1] 余州[2] 吴骐而 石定[2] 岳毅刚[1] 彭湃[2] 

机构地区：[1]桂林医学院附属医院烧伤整形美容科,广西桂林541004 [2]第四军医大学西京医院全军整形外科研究所,陕西西安710032

出　　处：《现代生物医学进展》2014年第35期6835-6838,6854,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81272118);国家自然科学基金面上项目(30973752)

摘　　要：目的:探讨不同剂量链脲佐菌素(Streptozotocin,STZ)联合高糖高脂饮食对2型糖尿病大鼠模型建立的影响。方法:90只8周龄SD雄性大鼠随机平均分为六组:普通饲料喂养+缓冲液组、高糖高脂饲料喂养+缓冲液(H.E组)、高糖高脂饲料喂养+35mg/kg链尿佐菌素组(H.E+35 mg/kg STZ组)、高糖高脂饲料喂养+45 mg/kg链尿佐菌素组(H.E+45 mg/kg STZ组)、高糖高脂饲料喂养+55 mg/kg链尿佐菌素组(H.E+55 mg/kg STZ组)及高糖高脂饲料喂养+65 mg/kg链尿佐菌素组(H.E+65 mg/kg STZ组),高糖高脂饲料喂养4周后诱导胰岛素抵抗,继之腹腔注射STZ,建立2型糖尿病大鼠模型。检测体重、胰岛素、空腹血糖、血脂、胰岛素敏感指数(ISI)。结果:与常规饮食组相比,高糖高脂饮食各组大鼠出现空腹血浆胰岛素(FINS)、空腹血糖(FBG)、血清甘油三脂(TG)、总胆固醇(TC)、游离脂肪酸(FFA)显著升高(P<0.01),ISI显著下降(P<0.01)。不同剂量STZ注射,H.E+45 mg/kg STZ组成模率最高且无自愈现象。结论:通过STZ腹腔注射联合高糖高脂饮食可成功复制出实验性2型糖尿病动物模型,45 mg/kg为STZ理想注射剂量。Objective： To investigate the effects of different doses of streptozotocin and high-glucose-high-fat diet on the establishment of type 2 diabetic rat model. Methods： Ninety 8-month-old SD male rats were randomly divided into six groups：Normal feeding group, high-glucose-high-fat feeding group（H.E group）, and high-glucose-high-fat＋STZ group which were intraperitoneal injected STZ with a dose of 35 mg/kg, 45 mg/kg, 55 mg/kg, and 65 mg/kg, respectively（H.E＋35/45/55/65 mg/kg_STZ group）. High-glucosehigh-fat diet feeding for four weeks to induce insulin resistance, then streptozotion was injected intraperitoneally to establish the type 2diabetes mellitus model. The changes of body weight, blood insulin level, fasting blood glucose level, blood lipid level, and insulin sensitivity index（ISI） were measured and statistically analyzed. Results： Compared with the control group, the fasting plasma insulin,fasting blood glucose, serum triglyceride（TG）, total cholesterol（TC） and free fatty acids（FFA） of H.E group and H.E ＋STZ group significantly increased（P〈0.01） and the ISI significantly decreased（P〈0.01）. From different doses of STZ injection, the 45 mg/kg STZ injection had the highest successful rate and effective stability to establish the models. Conclusion： High-glucose-high-fat diet combined with STZ injection can successfully replicate the experimental animal models of type 2 diabetes, and 45 mg/kg is an ideal dose for STZ injection.

关 键 词：2型糖尿病 动物模型 链脲佐菌素 

分 类 号：Q95-3[生物学—动物学] R587.1[医药卫生—内分泌]