金樱子提取液对糖尿病肾病大鼠的肾脏保护作用  

Renal Protective Effect of Extracts from Rosa Laevigata Mickx.on Diabetic Nephropathy Rats

作　　者：周钰娟[1,2,3] 廖前进[1,2] 罗玉平 许金华[3] 秦振启[3] 张秋菊[3] 何国平[5] 

机构地区：[1]湖南省肿瘤医院 [2]中南大学湘雅医学院附属肿瘤医院,湖南长沙410000 [3]南华大学生物化学与分子生物学教研室,湖南衡阳421001 [4]南华大学附属二医院药剂科,湖南衡阳421001 [5]中南大学护理学院,湖南长沙420008

出　　处：《现代生物医学进展》2014年第36期7019-7024,共6页Progress in Modern Biomedicine

基　　金：湖南省自然科学基金项目(13JJ4077);湖南省科技厅课题(2010FJ6025)

摘　　要：目的:研究金樱子提取液对糖尿病肾病(Diabetic Nephropathy,DN)大鼠的肾脏保护作用。方法:在高糖高脂饲料喂食SD(Sprague-Dawley)大白鼠的基础上腹腔注射链脲佐菌素(streptozotocin,STZ)诱导糖尿病肾病大鼠模型,随机分为糖尿病肾病模型组(DN组)和金樱子治疗组(DN+RLM组),同时另设正常对照组(NC组)和金樱子对照组(NC+RLM组)。检测金樱子提取液对各组大鼠血糖(fasting blood-glucose,FBG)、糖化血红蛋白(glycosylated haemoglobin,GHb)、24小时尿微量白蛋白和尿量、血尿素氮(BUN)、血肌酐(Scr)、胆固醇(TC)、甘油三酯(TG)及肾脏结构的影响。结果:与DN大鼠相比,糖尿病肾病大鼠经金樱子提取液治疗后,大鼠FBG、GHb水平、24 h尿微量白蛋白、24 h尿量、肾脏指数明显降低,血脂紊乱、肾功能损害以及DN肾脏病理明显改善,且无明显副作用。结论:金樱子提取液可明显降低DN大鼠血糖,改善DN大鼠血脂、肾功能紊乱及肾脏病理变化,对糖尿病大鼠肾脏具有较强的保护作用。Objective： To investigate the renal protective effect of extracts from Rosa Laevigata Mickx（RLM） on diabetic nephropathy rats. Methods： After fed with a high-fat/high-sucrose/high-cholesterol diet, SD rats were administered STZ in a single dose of 65mg/kg intraperitoneally（i.p.） to establish the diabetic nephropathy model, and then the diabetic nephropathy rats were randomly selected and divided into two groups as follows： Diabetic nephropathy model group（DN）, Diabetic nephropathy ＋ Rosa Laevigata Mickx.group（DN ＋ RLM, fed with extracts of RLM, 5 g/kg/d）. And normal SD rats were into divided two groups as follows： normal control group（NC）, and normal control ＋ Rosa Laevigata Michx. group（NC ＋ RLM, fed with extracts of RLM, 5 g/kg/d）. The fasting blood-glucose（FBG）, glycosylated haemoglobin（GHb）, urine microalbumin and urinevolume in 24 h, blood urea nitrogen（BUN）, serum creatinine（Scr）, total cholesterol（TC）, triglyceride（TG） and the renal pathology change were measured in every group. Results： Compared with the DN group, diabetic nephropathy rats which were treated with extracts of RLM, the disorder of FBG, GHb, urine microalbumin and urine volume in 24 h, dyslipidemia, renal dysfunction and the renal pathologicalchange were significantly ameliorated, and there was no noticeable side effects of RLM on the rats. Conclusion： RLM is able to ameliorate the disorder of plasma blood glucose, dyslipidemia,renal dysfunction and renal pathologicalchange, and has obvious renal protective effect on diabetic nephropathy rats.

关 键 词：金樱子 糖尿病肾病 肾功能 肾脏病理 

分 类 号：R587.1[医药卫生—内分泌] R285.5[医药卫生—内科学]