Plasma amino acid profiling of cancer patients with abnormal Savda based on high-performance liquid chromatography  

作　　者：Batur Mamtimin Kedena Bin Kong Ayshamgul Hasim Aynur Matsidik Mawlanjan Hizbulla Nazuk Kurbantay Halmurat Upur 

机构地区：[1]College of Pharmacy,Xinjiang Medical University [2]Department of Radiology,Beijing Tiantan Hospital,Capital Medical University [3]Basic Medical College,Xinjiang Medical University [4]College of Traditional Uyghur Medicine,Xinjiang Medical University

出　　处：《Journal of Traditional Chinese Medicine》2014年第5期560-565,共6页中医杂志（英文版）

基　　金：Supported by the China National Natural Science Foundation Program of Comparative Study of Abnormal Savda and Stasis-Phlegm Syndrome Tumor Patients Based on Metabonomics(No.81160459);the Postdoctoral Foundation of Xinjiang Medical University of Metabonomic Study of Abnormal Savda Tumor Patients(No.2012-27)

摘　　要：OBJECTIVE: To investigate metabolic signatures in plasma of cancer patients with abnormal Savda using plasma-free amino acid profiles, and to evaluate the diagnostic potential of these profiles for the detection and explanation of the mechanisms of different symptoms in traditional Uyghur medicine.METHODS: Plasma samples from cancer patients with abnormal Savda(n=85) or non-abnormal Savda(n=105) and a healthy control group(n=65)were analyzed using high-performance liquid chromatography(HPLC). Orthogonal projection to latent structures with discriminant analysis was used for the classification and prediction of abnormal Savda, and spectral profiles were subjected to Student's t-tests to assess statistical significance.RESULTS: Compared with the healthy group, the levels of aspartic acid, glutamate, glycine, histidine,arginine, threonine, alanine, proline, methionine,isoleucine, leucine and phenylalanine decreased significantly in plasma of cancer patients with abnormal Savda(all P<0.05). Serine, cystine, tyrosine,valine and lysine levels showed no significant differences(all P>0.05). Compared with non-abnormal Savda syndrome patients, abnormal Savda syndrome patients showed high concentrations of glutamate, serine, valine, isoleucine, leucine and phenylalanine(all P<0.05). The remaining plasma amino acids showed no significant differences(all P>0.05).CONCLUSION: Plasma-free amino acid profiling has the potential to assist in understanding and determining abnormal Savda. A HPLC-based metabonomic platform could be a powerful tool for the classification of symptoms in traditional medicine.OBJECTIVE： To investigate metabolic signatures in plasma of cancer patients with abnormal Savda using plasma-free amino acid profiles, and to evaluate the diagnostic potential of these profiles for the detection and explanation of the mechanisms of different symptoms in traditional Uyghur medicine.METHODS： Plasma samples from cancer patients with abnormal Savda（n=85） or non-abnormal Savda（n=105） and a healthy control group（n=65）were analyzed using high-performance liquid chromatography（HPLC）. Orthogonal projection to latent structures with discriminant analysis was used for the classification and prediction of abnormal Savda, and spectral profiles were subjected to Student＇s t-tests to assess statistical significance.RESULTS： Compared with the healthy group, the levels of aspartic acid, glutamate, glycine, histidine,arginine, threonine, alanine, proline, methionine,isoleucine, leucine and phenylalanine decreased significantly in plasma of cancer patients with abnormal Savda（all P〈0.05）. Serine, cystine, tyrosine,valine and lysine levels showed no significant differences（all P〉0.05）. Compared with non-abnormal Savda syndrome patients, abnormal Savda syndrome patients showed high concentrations of glutamate, serine, valine, isoleucine, leucine and phenylalanine（all P〈0.05）. The remaining plasma amino acids showed no significant differences（all P〉0.05）.CONCLUSION： Plasma-free amino acid profiling has the potential to assist in understanding and determining abnormal Savda. A HPLC-based metabonomic platform could be a powerful tool for the classification of symptoms in traditional medicine.

关 键 词：Amino acids Medicine  Uyghur tradi-tional Chromatography  High pressure liquid Ab-normal Savda 

分 类 号：O657.72[理学—分析化学] TS201.24[理学—化学]