Protective effect of Tanreqing injection on axon myelin damage in the brain of mouse model for experimental autoimmune encephalomyelitis  

作　　者：Yongping Fan Tao Yang Qi Zheng Lei Wang Chunxiao Yuan Ling Fang Kangning Li 

机构地区：[1]Department of Traditional Chinese Medicine,Beijing Tiantan Hospital,Capital Medical University [2]School of Traditional Chinese Medicine,Capital Medical University

出　　处：《Journal of Traditional Chinese Medicine》2014年第5期576-583,共8页中医杂志（英文版）

基　　金：Supported by the Research on the Effect of Catalpol on the OPCs' Proliferation and Differentiation(No.81173237);Research on the Impact of OPCs and Remyelination via the Method of Bushenyisu with EAE mice(No.81072765)of National Natural Science Foundation

摘　　要：OBJECTIVE: To evaluate the effect of Tanreqing injection on axon myelin in the mouse brain of experimental autoimmune encephalomyelitis(EAE).METHODS: An EAE model was established by myelin oligodendrocyte glycoprotein(MOG)35-55 immunization in C57BL/6 mice. Mice were randomly divided into the following groups: normal, model,prednisone acetate(PA)(6 mg/kg), Tanreqing high dose(5.14 m L/kg), Tanreqing low dose(2.57 m L/kg). On the day of immunization, both Tanreqing groups were treated by intraperitoneal injection,with the PA group treated by intragastrical perfusion after T cell response, and the other groups treated with saline. Changes in body weight, neurological deficit score, incidence rate, mortality rate,and course of disease were observed for all mice.Brain tissue was isolated and stained with hematoxylin-eosin, and pathological investigations performed to evaluate axon myelin damage by transmission electron microscopy(TEM). Myelin basic protein and microtubule associated protein-2 were analyzed by immunohistochemistry.RESULTS: Tanreqing injection significantly prolonged EAE latency and decreased the neurological deficit score, alleviated infiltration of inflammatory cells in the focus area, up-regulated hippocampal MBP expression at the acute stage and the remission stage, and increased microtubule associated protein-2 expression in the EAE brain to varying degrees in the acute stage. TEM analysis indicated that Tanreqing injection alleviates myelin damage in the EAE mouse and maintains the integrity of circular layer structures and alleviates axon mitochondrial swelling.CONCLUSION: Tanreqing injection alleviates EAE symptoms.OBJECTIVE： To evaluate the effect of Tanreqing injection on axon myelin in the mouse brain of experimental autoimmune encephalomyelitis（EAE）.METHODS： An EAE model was established by myelin oligodendrocyte glycoprotein（MOG）35-55 immunization in C57BL/6 mice. Mice were randomly divided into the following groups： normal, model,prednisone acetate（PA）（6 mg/kg）, Tanreqing high dose（5.14 m L/kg）, Tanreqing low dose（2.57 m L/kg）. On the day of immunization, both Tanreqing groups were treated by intraperitoneal injection,with the PA group treated by intragastrical perfusion after T cell response, and the other groups treated with saline. Changes in body weight, neurological deficit score, incidence rate, mortality rate,and course of disease were observed for all mice.Brain tissue was isolated and stained with hematoxylin-eosin, and pathological investigations performed to evaluate axon myelin damage by transmission electron microscopy（TEM）. Myelin basic protein and microtubule associated protein-2 were analyzed by immunohistochemistry.RESULTS： Tanreqing injection significantly prolonged EAE latency and decreased the neurological deficit score, alleviated infiltration of inflammatory cells in the focus area, up-regulated hippocampal MBP expression at the acute stage and the remission stage, and increased microtubule associated protein-2 expression in the EAE brain to varying degrees in the acute stage. TEM analysis indicated that Tanreqing injection alleviates myelin damage in the EAE mouse and maintains the integrity of circular layer structures and alleviates axon mitochondrial swelling.CONCLUSION： Tanreqing injection alleviates EAE symptoms.

关 键 词：Multiple sclerosis Encephalomyelitis autoimmune  experimental Tanreqing injection Myelin basic protein Microtubule-associated pro-teins 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]