二甲氨基乙醇和复方氨基酸溶液对衰老大鼠皮肤Ⅰ型和Ⅲ型胶原蛋白mRNA表达的影响  

Effect of dimethylaminoethano and compound amino acid on collagens Ⅰ and Ⅲ mRNA expression in theskin of D-galactose induced aging model in rats

作　　者：刘肃[1,2] 孙瑛[3] 赵彩玲[4] 柳大烈[5] 

机构地区：[1]南方医科大学珠江医院整形外科,广州510280 [2]青岛大学医学院附属医院整形美容科 [3]北京黄寺美容外科医院皮肤科 [4]山东省莱芜市人民医院烧伤整形科 [5]南方医科大学珠江医院整形外科

出　　处：《中华医学美学美容杂志》2014年第6期455-459,共5页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的依据中胚层疗法理念，观察真皮内注射二甲氨基乙醇（Dimethylaminoethano，DMAE）和复方氨基酸溶液（compound amino acid injection，AA）对D-半乳糖（D-galactose，D-gal）诱导的化学性衰老大鼠皮肤Ⅰ型和Ⅲ型胶原蛋白mRNA表达的影响。方法80只Wistar大鼠随机分为正常对照组、衰老对照组和衰老治疗组。衰老治疗组从注射D-gal后第18天开始，在大鼠两侧臀部皮肤分别皮内注射0．2％DMAE＋AA、0．1％DMAE＋AA、0．2％DMAE、0．1％DMAE、AA、生理盐水（NS），1次／周，连续4周。观察各组皮肤组织学变化、真皮厚度、羟脯氨酸（Hydroxyproline，Hyp）、Ⅰ型和Ⅲ型胶原蛋白mRNA和成纤维细胞增殖细胞核抗体（proliferating cell nuclea antigen，PCNA）表达水平的变化。结果真皮内注射0．1％DMAE＋AA、0．2％DMAE＋AA可使衰老大鼠真皮厚度、Hyp、Ⅰ型和Ⅲ型胶原蛋白mRNA表达水平显著增加（P〈O．05），而对PCNA表达无显著影响（P〉O．05）。结论真皮内注射0．2％DMAE＋AA、0．1％DMAE＋AA可促进衰老大鼠皮肤Ⅰ型和Ⅲ型胶原蛋白合成，显著增加真皮厚度和胶原蛋白含量，具有一定延缓皮肤衰老的作用。Objective To study the effect of dimethylaminoethano （DMAE） and compound ami- no acid injection （AA） by mesotherapy on collagen Ⅰand collagen Ⅲ mRNA expression levels of D- galactose-induced chemical skin aging rats. Methods 80 rats were randomly divided into individual ex- perimental group. At 18 days after D-gal induction, rats of aging treatment groups were treated with intradermal microinjection of 0. 2% DMAE＋AA, 0. 1% DMAE＋ AA, 0. 2% DMAE, 0. 1% DMAE, AA, and saline, respectively, once a week for 4 weeks. At 42 days after treatment, the skin wounds were harvested. HE, PCNA, hydroxyproline and collagen I and collagen m mRNA expres- sion levels of every group skins were detected. Results Dermal thickness, hydroxyproline content and collagen type I and III mRNA expression levels of 0.1% DMAE＋AA and 0.2% DMAE＋AA groups were significantly improved （P〈0.05）, but PCNA expression of sham control group was significantly higher than all of aging groups （P〉0.05）. Conclusions Local co-injection of DMAE and compound AA directly into targeted tissue under mesotherapy has marked anti-aging effects on D-galactose in- duced skin aging model of rat by increasing the dermal thickness, collagen content and acceleration of collagen synthesis.

关 键 词：中胚层疗法 二甲氨基乙醇 复方氨基酸 抗衰老 Ⅰ型和Ⅲ型胶原蛋白 成纤维细胞增殖细胞核抗体 D-半乳糖 

分 类 号：R751[医药卫生—皮肤病学与性病学]