位点特异性重组系统及其在植物转基因研究中的应用  

Site-specific Recombination System and Its Application in Plant Genetic Engineering

作　　者：吴花拉 张严玲[1] 罗旭[1] 葛飞[1] 潘光堂[1] 沈亚欧[1] 

机构地区：[1]四川农业大学玉米研究所农业部西南玉米生物学与遗传育种重点实验室,成都611130

出　　处：《中国生物工程杂志》2014年第11期107-118,共12页China Biotechnology

基　　金：国家转基因生物新品种培育科技重大专项资助项目(2014ZX08003003)

摘　　要：随着植物转基因研究的不断深入,对基因重组系统提出了新的要求。位点特异性重组系统具有高效、精确等的优点,在植物基因工程领域的应用越来越广泛。对常用的三类位点特异性重组系统的作用机制、优缺点及其应用进展进行了全面的综述,期望为植物转基因研究提供技术参考。对于目前研究较为热点的基因编辑技术CRISPR-Cas系统作了简要概述。Gene recombination system is required to meet the rapid development of plant gene engineering. With the advantages of highprecision and efficiency, site-specific recombination systems havebeen widely used in the field of genetic engineering. The mechanism, advantages and disadvantages, and application of three site- specific recombination systems are summarized in the article, which are expected to contribute to the researches on plant transgenic engineering. Meanwhile, the current research hotspot for gene editing technology, the CRISPR- Cas system is introduced briefly in this paper.

关 键 词：位点特异性重组系统 CRE/LOX FLP/FRT R/RS CRISPR-Cas 

分 类 号：Q78[生物学—分子生物学]