Proteomic analysis of BmN cells （Bombyx morl） in response to infection with Nosema bombycis  

作　　者：Xinyi He Xiangkang He Han Liu Mingqian Li Shunfeng Cai Zhangwuke Fu Xingmeng Lu 

机构地区：[1]Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

出　　处：《Acta Biochimica et Biophysica Sinica》2014年第11期982-990,共9页生物化学与生物物理学报（英文版）

摘　　要：Nosema bombycis （N. bombycis, Nb） is an obligate intracel- lular parasite, which can cause pebrine disease in the silk- worm. To investigate the effects ofN. bombycis infection on the host cells, proteomes from BraN cells that had or had not been infected with N. bombycis at different infection stages were characterized with two-dimensional gel electro- phoresis and MALDI-TOF/TOF mass spectrometry, which identified 24 differentially expressed host proteins with sig- nificant intensity differences （P 〈 0.05） at least at one time point in mock- and N. bombycis infected cells. Notably, gene ontology analyses showed that these proteins are involved in many important biological reactions. During the infec- tion phase, proteins involved in energy metabolism and oxidative stress had up-regulated expression. Two proteins participated in ubiquitin-dependent protein catabolic pro- cess had down-regulated expression. Quantitative real-time polymerase chain reaction was used to analyze the tran- scriptional profiles of these identified proteins. Taken to- gether, the abundance changes, putative functions, and participation in biological reactions for the identified pro- teins produce a host-responsive protein model in N. bombycis- infected BmN cells. These findings further our knowledge about the effect of energy defect parasites on the host cells.Nosema bombycis （N. bombycis, Nb） is an obligate intracel- lular parasite, which can cause pebrine disease in the silk- worm. To investigate the effects ofN. bombycis infection on the host cells, proteomes from BraN cells that had or had not been infected with N. bombycis at different infection stages were characterized with two-dimensional gel electro- phoresis and MALDI-TOF/TOF mass spectrometry, which identified 24 differentially expressed host proteins with sig- nificant intensity differences （P 〈 0.05） at least at one time point in mock- and N. bombycis infected cells. Notably, gene ontology analyses showed that these proteins are involved in many important biological reactions. During the infec- tion phase, proteins involved in energy metabolism and oxidative stress had up-regulated expression. Two proteins participated in ubiquitin-dependent protein catabolic pro- cess had down-regulated expression. Quantitative real-time polymerase chain reaction was used to analyze the tran- scriptional profiles of these identified proteins. Taken to- gether, the abundance changes, putative functions, and participation in biological reactions for the identified pro- teins produce a host-responsive protein model in N. bombycis- infected BmN cells. These findings further our knowledge about the effect of energy defect parasites on the host cells.

关 键 词：microsporida Nosema bombycis 2D-PAGE energy metabolism oxidative stress 

分 类 号：S884.21[农业科学—特种经济动物饲养] Q51[农业科学—畜牧兽医]