海马齿甜菜碱醛脱氢酶基因在大肠杆菌中表达  

Expression of Recombinant Betaine Aldehyde Dehydrogenase of Sesuvium portulacastrum in Escherichia coli

作　　者：杨成龙[1,2,3] 刘姣[1] 周扬[1,3,4] 李瑞梅[1] 段瑞军[1] 符少萍[1] 胡新文[3] 郭建春[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,农业部热带作物生物学与遗传资源利用重点实验室,海口571101 [2]贵州省亚热带作物研究所,兴义562400 [3]海南大学农学院,海口570228 [4]华中农业大学植物科学技术学院,武汉430070

出　　处：《分子植物育种》2014年第6期1275-1280,共6页Molecular Plant Breeding

基　　金：海南省重大科技专项(ZDZX2013023-1);中国热带农业科学院农业杰出人才项目(CATAS-1630052014004);中央级公益性科研院所基本科研业务专项基金(ITBB130302)共同资助

摘　　要：本研究利用从海马齿中克隆到的甜菜碱醛脱氢酶基因构建到高效表达载体p GEX-4T-1上,构建重组载体p GEX-4T-Sp BADH,并对GST-Sp BADH融合表达的IPTG诱导浓度、诱导不同温度、不同菌液浓度和诱导时间等条件进行优化。研究结果表明:随诱导时间增长GST-Sp BADH融合蛋白表达量提高,在37℃时,OD为0.6左右,诱导5 h GST-Sp BADH融合蛋白的表达量达到最大,在0.2 mmol/L IPTG浓度下,可以有效诱导GST-Sp BADH融合蛋白的表达。本研究结果以期解析Sp BADH基因的功能以及在海马齿抗盐过程中的作用机理。A full-length betaine aldehyde dehydrogenase gene sequence ofSesuvium portulaeastrum was cloned into the high expression vector pGEX-4T-1, and named pGEX-4T-SpBA DH. The GST-SpBADH fusion protein was expressed induced by IPTG. The expression conditions such as IPTG concentration, the bacterium concen- tration, induction time and temperature were optimized. The results showed that the expression level of GST- SpBADH was increased by accompanying with the induction time. In the condition of 37℃, OD600 is 0.6 and cultured 5 hours, GST-SpBADH fusion protein reached the highest expression. 0.2 mmol/L concentration of IPTG can induce GST-SpBADH expression effectively in Eseherichia coli expression system. The aim of this research was to analyze the function and salt tolerance mechanism of SpBA DH gene.

关 键 词：海马齿 甜菜碱醛脱氢酶 原核表达 

分 类 号：Q943.2[生物学—植物学]