维药异常黑胆质成熟剂对增生性瘢痕及转化生长因子β/Smad信号通路的影响  

Effect of Uighur medicine abnormal savda munzip on hypertrophic scar and transforming growth factor beta/Smad signal transduction pathway

作　　者：李楠[1] 孔孟龙 马涛[1] 李加辅 马少林[1] 

机构地区：[1]新疆医科大学第一附属医院烧伤整形科,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究》2014年第46期7391-7395,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81260291)~~

摘　　要：背景:近年来,研究发现异常黑胆质成熟剂具有良好的减轻瘢痕增生的作用,但具体机制尚未清楚,考虑异常黑胆质成熟剂是否是通过影响转化生长因子β/Smad信号通路的表达来抑制瘢痕增生的。目的:验证维药异常黑胆质成熟剂对体外培养的人增生性瘢痕成纤维细胞增殖及对转化生长因子β/Smad信号通路的影响。方法:用组织块法培养人增生性瘢痕成纤维细胞,将成纤维细胞分为6组:1空白对照组:加入等体积的培养液。2异常黑胆质成熟剂处理组:分成5组,分别加入质量浓度为0.2,0.4,0.6,0.8,1.0 g/L的异常黑胆质成熟剂。对各组细胞采用相应药物进行干预,采用MTT、流式细胞术、免疫细胞化学方法检测其在正常状况和应用维药异常黑胆质成熟剂后细胞增殖与转化生长因子β/Smad信号通路的改变。结果与结论:与空白对照组相比,异常黑胆质成熟剂对增生性瘢痕来源成纤维细胞的增殖有抑制作用,且在0.2-0.8 g/L质量浓度范围之间,随着质量浓度增加,抑制作用呈增强趋势。细胞免疫化学结果测定表明,异常黑胆质成熟剂干预后减少了成纤维细胞中转化生长因子β1的含量,增加了细胞中Smad7的含量。提示异常黑胆质成熟剂抑制瘢痕的作用可能是通过转化生长因子β/Smad通路,使成纤维细胞增殖受阻而发挥作用的。BACKGROUND： Previous observations have suggested that abnormal savda munziq has a possible anti-scaring effect. However, the precise pathological mechanism still remains unknown. Hero, it is considered whether abnormal savda munziq can alleviate the formation of hypertrophic scar by affecting the expression of transform growth factor β （TGF-β）/Smad signal transduction pathway. OBJECTIVE： To evaluate the in vitro effect of abnormal savda munziq on the proliferation and TGF-β/Smad signal transduction pathway in hypertrophic scar fibroblasts. METHODS： Hypertrophic scar fibroblasts were divided into six groups to receive different treatments as group A （blank control group）, groups B-E （0.2, 0.4, 0.6, 0.8, 1.0 g/L abnormal savda munziq）. Each group contained six specimens. After intervention, the MTT test, flow cytometry and immunocytochemistry methods were used to detect the alteration of cell proliferation and TGF-β/Smad signal transduction pathway. RESULTS AND CONCLUSION： The inhibition effect of abnormal savda munziq （0.2-0.8 g/L） on the fibroblasts enhanced in a concentration-dependent manner than the blank control group （P 〈 0.05）. The immunocytochemistry results showed that the expression of Smad7 increased while expression of TGF-β1 decreased in hypertrophic, scar fibroblasts with abnormal savda munziq. These findings suggest that the reduction on the proliferation caused by abnormal savda munziq could be attributed to its effects on TGF-β/Smad signal transduction pathway.Abnormal savda munziq could be used as an effective drug for treatment of hypertrophic scar.

关 键 词：组织构建 组织工程 异常黑胆质成熟剂 增生性瘢痕 成纤维细胞 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]