激光对炎症牙髓中碱性成纤维细胞生长因子表达的影响  

Effect of Nd:YAG laser on the expression of basic fibroblast growth factor in inflammatory dental pulp

作　　者：毛小泉[1] 郁豆[1] 程亚楠[1] 王月燕[1] 刘德达 

机构地区：[1]中南大学湘雅医学院附属海口医院口腔中心.海南省口腔医学中心,海南省海口市570208

出　　处：《中国组织工程研究》2014年第46期7401-7405,共5页Chinese Journal of Tissue Engineering Research

基　　金：海口市重点科技计划项目(2011-0136)~~

摘　　要：背景:激光应用于口腔临床主要用于龋齿的早期诊断、龋坏组织的清除、活髓切断术牙髓止血、牙本质过敏治疗、感染的根管消毒、牙周炎治疗。目的:观察炎症牙髓使用激光处理后碱性成纤维细胞生长因子的变化。方法:5只成年健康beagle犬,每只随机选择6颗牙齿,分为3组,每组10颗,分别使用激光、生理盐水、庆大霉素处理,于术后第1,2,3天用纸尖收集牙髓分泌物检测碱性成纤维细胞生长因子的含量,并进行牙髓苏木精-伊红染色和免疫组织化学检查。结果与结论:生理盐水处理组、庆大霉素处理组及激光处理组第3天碱性成纤维细胞生长因子表达量均明显多于第1天和第2天,第1天和第2天比较差异无显著性意义,3种处理方法之间比较差异无显著性意义。牙髓苏木精-伊红染色和免疫组织学检查显示,髓腔血管状况良好,牙髓排列紧密良好。结果表明,激光治疗对炎症牙髓中碱性成纤维细胞生长因子的释放没有影响。提示激光在口腔临床上的应用是安全、方便、有效的,不会对炎症牙髓组织造成损伤。BACKGROUND：The laser applied in the clinical oral medicine is mainly for early diagnosis of caries, removal of carious tissue, pulpotomy for pulp bleeding, dentin hypersensitivity treatment, disinfection of infected root canals, and periodontitis treatment. OBJECTIVE：To explore the changes of basic fibroblast growth factor in inflammatory pulp after laser treatment. METHODS：Five healthy adult beagle dogs were enroled, and six teeth were randomly selected from each dog. Then, these teeth were randomly divided into three groups, 10 teeth in a group, and they were treated with laser irradiation, saline and gentamicin irrigation respectively. At days 1, 2, 3 after operation, exudates from the pulp were colected for detection of basic fibroblast growth factor expression. Meanwhile, the dental pulp was detected by hematoxylin-eosin staining and immunohistochemistry method. RESULTS AND CONCLUSION：In the al three groups, the expression of basic fibroblast growth factor at day 3 was significantly higher than that at days 1 and 2, and there were no differences between days 1 and 2. Moreover, these three groups also showed no significant differences. Under hematoxylin-eosin and immunohistochemical staining, blood vessels in the pulp cavity were in good condition and the pulp arranged tightly. These findings suggest that the laser is safe, convenient and effective for oral clinical application, and has no injury to the inflammatory pulp.

关 键 词：组织构建 组织工程 激光 牙髓炎 碱性成纤维细胞生长因子 

分 类 号：R318[医药卫生—生物医学工程]