不同浓度许旺细胞与聚乳酸-聚羟基乙酸共聚物修复损伤周围神经的比较  

Polylactic-co-glycolic acid complex with different concentrations of Schwann cells for peripheral nerve regeneration

作　　者：孙焕伟[1] 张铁慧[1] 由欣岩 任远飞[1] 钟声[1] 

机构地区：[1]声大连市中心医院手足外科,辽宁省大连市116033

出　　处：《中国组织工程研究》2014年第47期7579-7584,共6页Chinese Journal of Tissue Engineering Research

基　　金：大连市卫生局课题~~

摘　　要：背景:有研究表明,许旺细胞神经移植复合体具有极强修复自体神经缺损作用,并且许旺细胞在神经再生过程中发挥至关重要的作用。目的:比较不同浓度许旺细胞神经移植复合体修复自体神经缺损时周围神经的再生效果。方法:建立坐骨神经缺损模型大鼠。原代培养大鼠许旺细胞,构建聚乳酸-聚羟基乙酸共聚物管-细胞外基质凝胶-许旺细胞神经移植复合体修复坐骨神经缺损模型大鼠。按许旺细胞浓度的不同分为105,106,107,108,109 L-1浓度组,对照组不含许旺细胞。分别于建模后3,6和12周,行运动神经传导速度的测定。建模后12周各组胫骨前肌湿质量测量和组织学观察。结果与结论:建模后3,6和12周,含许旺细胞各浓度的神经移植复合体组各时间点神经传导速率均高于对照组(P<0.01),其中108 L-1组运动神经传导速度优于其他各浓度组(P<0.05)。建模后12周,大鼠胫骨前肌苏木精-伊红染色显示,各浓度许旺细胞神经移植复合体组正常肌纤维数均多于对照组(P<0.05)。其中许旺细胞浓度108,109 L-1浓度组胫骨前肌形态恢复较好,肌纤维细条样、波浪状,同向而行,长短、粗细及疏密大致一致。结果证实,108 L-1许旺细胞神经聚乳酸-聚羟基乙酸共聚物移植复合体对缺损坐骨神经再生的促进作用较好。BACKGROUND：Studies have shown that nerve grafts with Schwann cels can repair peripheral nerve defect and Schwann cels have an important role in nerve regeneration. OBJECTIVE： To observe the rehabilitation status of neutral function after sciatic nerve injury in rats bridged by nerve grafts with Schwann cels. METHODS： A rat model of sciatic nerve injury was established, and schwann cels were primarily cultured. Then, the rat model was repaired with polylactic-co-glycolic acid copolymer-extracelular matrix gel-Schwann cels complex. According to different concentrations of Schwann cels, there were five cel groups from 105/L to 109 RESULTS AND CONCLUSION： The nerve conduction velocities in the cel groups were al higher than that in the control group at 3, 6, 12 weeks after modeling （P 〈 0.01）, and it was highest in the 10 and a control group. The nerve conduction velocity was detected respectively at 3, 6, 12 weeks after modeling; the e tibialis anterior muscle gravity was detected and histological observation was done at 12 weeks. 8/L （P 〈 0.05）. At 12 weeks, hematoxylin-eosin staining of the tibialis anterior muscle showed that the number of normal muscle fibers was higher in the cel groups than the control group （P 〈 0.05）. In the 108/L and 109 and had similar length, thickness and density. These findings indicate that polylactic-co-glycolic acid complex with 10/L groups, the morphology of tibialis anterior muscle recovered wel; the muscle fibers were in strip-like and wavy shapes, grew in the same direction, 8/L Schwann cels is better to promote sciatic nerve regeneration.

关 键 词：生物材料 材料相容性 聚乳酸-聚羟基乙酸共聚物 周围神经 神经再生 坐骨神经 许旺细胞 神经缺损 神经移植物 最佳浓度 人工材料 神经修复 

分 类 号：R318[医药卫生—生物医学工程]