冻干法制备壳聚糖管状支架的理化性质  

Properties of a chitosan tubular scaffold prepared by lyophilization

作　　者：吴俞萱 马昊 傅重洋[1] 蒋华军[1] 陶弘 曲巍[1] 

机构地区：[1]大连医科大学附属第一医院,辽宁省大连市116021 [2]大连医科大学附属第三医院,辽宁省大连市116044 [3]无锡市第九人民医院,江苏省无锡市214062

出　　处：《中国组织工程研究》2014年第47期7603-7607,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(30973060)~~

摘　　要：背景:冻干法的原理是将材料溶液冷冻塑性后于真空状态下升华溶剂,保留溶质,从而制作出具有孔隙结构支架的方法。目的:利用冻干法制备壳聚糖管状支架材料,研究管状支架的理化性质。方法:采用冻干法制备壳聚糖管状材料,直接观察材料的自然形态,电镜下观察材料的微观结构。将壳聚糖聚糖管状材料分别放入PBS和纯水中各50 d,放入胰酶液体中1 d,同时将其植入SD乳鼠肌肉及背部皮下30 d,观察材料降解率,计算材料的孔隙率。利用拉伸力学仪器测定壳聚糖管状材料在干燥时和浸水后的拉伸力学,并测量干燥时的拉伸率,利用压力计测量壳聚糖管状材料在干燥和浸水后的抗压能力。结果与结论:壳聚糖管状材料外部形态呈标准管状,电镜下可见材料为大小不同的孔隙组成,孔隙较均匀分布,孔隙大小为50-200μm。壳聚糖管状材料在PBS、纯水、胰酶及小鼠体内的降解率分别为(5.33±0.12)%,(11.26±0.15)%,0.012%,(35.2±3.7)%,材料的孔隙率为(97.5±1.5)%。壳聚糖管状材料干燥状态下的断裂强度与抗压能力均高于浸水状态(P<0.05)。表明冻干法制备的壳聚糖管状材料具有良好的降解率及孔隙率,同时也具有较好的拉伸力学及抗压能力。BACKGROUND：The principle of lyophilization is to sublimate the solvent of frozen materials in vacuum and retain the solute, thus making a pore structure. OBJECTIVE： To produce a chitosan tubular scaffold by lyophilization, and to test its physicochemical properties. METHODS： The chitosan tubular material was prepared by lyophilization method, folowed by gross observation and electron microscopic observation. The chitosan tubular material samples were placed into PBS solution and pure water for 50 days, respectively, and then immersed in trypsin liquid for 1 day folowed by embedded into the muscle and dorsal skin of neonatal Sprague-Dawley rats for 30 days. The degradation rate and porosity of the material were observed and calculated. The breaking strength and compressive strength of the material were determined both under drying and soaking conditions using tensile instrument and pressure meter, respectively. RESULTS AND CONCLUSION：The external form of the chitosan tubular material was normaly tubular. Underthe electron microscope, it was composited by different size pores, and the pore size was 50-200μm. The degradation rates of the material were （5.33±0.12）% in PBS, （11.26±0.15） in water, 0.012% in the trypsin liquid and （35.2±3.7）in vivo. The porosity rate was （97.5±1.5）%. The breaking strength and compressive strength of the material was higher under the drying state than under the soaking state （P 〈 0.05）. These findings indicate that the lyophilization method can produce the chitosan tubular material with good porosity rate and degradation rate as wel as good tensile ability and compressive capability.

关 键 词：生物材料 材料相容性 壳聚糖 冻干法 降解率 孔隙率 拉伸力学 抗压能力 电镜 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]