有氧运动干预自发性高血压模型大鼠主动脉血管内皮细胞c-Src mRNA表达和c-Src的活性  

Aerobic exercise affects c-Src m RNA expression and c-Src activity in aortic vascular endothelial cells of spontaneous hypertensive rat models

作　　者：任彩玲[1] 齐洁[2] 张钧[2] 

机构地区：[1]赣南医学院,江西省赣州市341000 [2]上海师范大学,上海市200234

出　　处：《中国组织工程研究》2014年第49期7943-7947,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:原癌基因c-Src在调节高血压等心血管系统疾病中起着重要的作用,目前尚未看到有关运动干预影响主动脉血管内皮细胞c-Src的表达和活来调节高血压的研究。目的:观察有氧运动对自发性高血压大鼠主动脉血管内皮细胞c-Src m RNA表达和c-Src活性的影响。方法:8只雄性Wistar大鼠作为正常对照组,16只自发性高血压大鼠随机分为自发性高血压组8只,自发性高血压运动组8只。自发性高血压运动组每天进行90 min无负重有氧游泳运动,6 d/周,共8周;正常对照组和自发性高血压组大鼠不做游泳运动。每周测定大鼠血压1次,8周后,测定各组大鼠主动脉血管内皮细胞c-Src m RNA表达和c-Src活性。结果与结论:与自发性高血压组相比,自发性高血压运动组血压明显降低,主动脉血管内皮细胞c-Src m RNA表达和c-Src活性明显升高。与正常对照组相比,自发性高血压运动组大鼠主动脉血管内皮细胞c-Src活性和c-Src m RNA表达高于正常对照组和自发性高血压组(P<0.01)。结果提示有氧运动可以促进自发性高血压大鼠主动脉血管内皮细胞c-Src活性和c-Src m RNA表达的增加。BACKGROUND：Proto-oncogene c-Src plays an important role in regulating cardiovascular diseases such as hypertension. At present, there were no studies concerning exercise intervention effects on c-Src expression in aortic endothelial cels so as to regulate hypertension. OBJECTIVE： To observe the effects of aerobic exercise on c-Src mRNA expression and c-Src activity in the aorta blood vessel endothelial cels of spontaneous hypertensive rats. METHODS： A total of 8 male Wistar rats were considered as normal control group. Sixteen spontaneous hypertensive rats were randomly assigned to 8 rats as spontaneous hypertension group and 8 rats as spontaneous hypertension exercise group. Rats in the spontaneous hypertension exercise group carried on 90 minutes unloaded aerobic swimming every day, 6 days a week, for 8 weeks. The rats in the normal control group and spontaneous hypertension group did not swim. Blood pressure of rats was measured once a week. 8 weeks later, the c-Src mRNA expression and c-Src activity were determined in aortic vascular endothelial cels of rats in each group. RESULTS AND CONCLUSION： Compared with spontaneous hypertension group, blood pressure was lower, but c-Src mRNA expression and c-Src activity were significantly higher in the spontaneous hypertension exercise group. The c-Src activity and c-Src mRNA expression were higher in the spontaneous hypertension exercise group than normal control group and spontaneous hypertension group （P 〈 0.01）. Results indicated that aerobic exercise can promote the increase in c-Src activity and c-Src mRNA expression in aortic endothelial cels of spontaneous hypertensive rats.

关 键 词：实验动物 组织工程 c-Src活性 高血压 运动 

分 类 号：R318[医药卫生—生物医学工程]