回肠代膀胱家兔模型制作中不同的麻醉药物  

Anesthetics in establishment of rabbit models of ileal neobladder

作　　者：沈俭[1] 黄春霞[2] 余云兰 刘洪新[1] 程志军 

机构地区：[1]上海交通大学医学院附属新华医院崇明分院泌尿科,上海市202150 [2]上海交通大学医学院附属新华医院崇明分院麻醉科,上海市202150 [3]上海市崇明新华癌痛转化研究所,上海市202150

出　　处：《中国组织工程研究》2014年第49期7990-7994,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:家兔回肠代膀胱模型制作对麻醉要求较高,需要手术野清晰,术毕恢复迅速,麻醉药物及方法的选择不影响肠道平滑肌的蠕动。目的:比较水合氯醛和乌拉坦对家兔回肠代膀胱模型制作的麻醉效果。方法:18只家兔随机等分为乌拉坦组和水合氯醛组,分别用体积分数10%水合氯醛2 m L/kg和体积分数20%乌拉坦5 m L/kg实施耳缘静脉麻醉后,于原膀胱外1 cm处离断双输尿管,种植于回盲部10 cm处切取的15 cm肠段,建立回肠代膀胱模型。结果与结论:2组家兔麻醉后心率均较麻醉前出现明显下降(P≤0.05),但2组间差异无显著性意义(P>0.05)。与水合氯醛组相比,乌拉坦组兔被麻醉的持续时间、苏醒时间过长,死亡率高。说明水合氯醛是适合家兔回肠代膀胱模型制作的安全长效的麻醉剂。BACKGROUND：The anesthesia process is required strict in establishing rabbit models of ileal neobladder. It is required for cleaning operation field, quick postoperative recovery, and the choice of anesthetics and anesthesia does not influence smooth muscle peristalsis. OBJECTIVE：To compare the anesthetic effects between urethane and chloral hydrate in rabbits with ileal neobladder. METHODS：Eighteen rabbits were randomly assigned to urethane and chloral hydrate groups, and were respectively anesthetized with 20% urethane 5 mL/kg and 10% chloral hydrate 2 mL/kg through the ear vein. Double ureter was dissociated at 1 cm outside original bladder, and 15 cm-intestine obtained from 10 cm of ileocecal junction was implanted so as to establish models of ileal neobladder. RESULTS AND CONCLUSION：Heart rates were significantly lower compared with these before anesthesia in al cases （P≤ 0.05）, but no differences were detected between two groups （P 〉 0.05）. Compared with the chloral hydrate group, the maintain time and recovery time were longer and the mortality rate was higher in the urethane group. Above data indicated that chloral hydrate is a safe long-acting anesthetic in establishing rabbit models of ileal neobladder.

关 键 词：实验动物 组织工程 回肠代膀胱 动物模型 麻醉 水合氯醛 乌拉坦 围术期 麻醉剂 

分 类 号：R318[医药卫生—生物医学工程]