布洛芬抑制人NSCLC细胞株NCI-H460和乳腺癌细胞株SKBR3增殖和转移的机制探讨  

Ibuprofen suppresses proliferation and metastasis of human non-small cell lung cancer cell line NCI-H460 and breast cancer cell line SKBR3

作　　者：祝丽双[1] 王浩[2] 陈佳[1] 刘畅[1] 陆慧琪[1] 韩焕兴[1] 

机构地区：[1]第二军医大学长征医院转化医学中心,上海200433 [2]第二军医大学长征医院眼科,上海200003

出　　处：《第二军医大学学报》2014年第12期1327-1332,共6页Academic Journal of Second Military Medical University

基　　金：国家科技部国际合作专项(OS2014ZR0042)~~

摘　　要：目的阐述布洛芬在人非小细胞肺癌(non-small cell lung caner,NSCLC)细胞株NCI-H460和乳腺癌细胞株SKBR3中的抗增殖和抑制迁移作用,并对其机制进行探讨。方法运用MTT法检测布洛芬作用下两种不同肿瘤细胞株的细胞增殖抑制情况;运用Transwell方法检测布洛芬作用下两种不同肿瘤细胞株的迁移抑制情况;运用real-time聚合酶链反应(polymerase chain reaction,PCR)和蛋白质印迹方法分析肿瘤细胞survivin和E-cadherin的mRNA和蛋白表达情况。结果 MTT法检测结果显示,布洛芬降低了两种肿瘤细胞株的增殖,并且随着布洛芬药物浓度增高抑制增殖作用增强;Transwell显示,2mmol/L布洛芬作用于两种肿瘤细胞株12h,能够抑制肿瘤细胞株的转移;RT-PCR和蛋白质印迹分析显示,布洛芬能够导致肿瘤细胞survivin的表达降低,使E-cadherin表达增高。结论布洛芬体外能够显著抑制人非小细胞肺癌细胞株NCI-H460和乳腺癌细胞株SKBR3增殖和迁移,这一抑制作用与survivin表达降低和E-cadherin表达增高有关。Objective To investigate the inhibitory effect of ibuprofen on proliferation and migration of non-small cell lung cancer （NSCLC） cell line NCI-H460 and breast cancer cell line SKBR3, and to discuss the possible mechanism. Methods MTT method was used to detect the proliferation inhibition of the two cancer cell lines after ibuprofen treatment for 24, 48, and 72 h; and Transwell assay was used to study the cell migration in vitro. Real-time PCR and Western blotting analysis were used to detect the mRNA and protein expression of survivin and E-cadherin after ibuprofen treatment. Results MTT assay showed that ibuprofen treatment decreased cell growth in the two cell lines in a dose-dependent manner. Ibuprofen treatment （2 mmol/L, 12 h） significantly inhibited the cell metastasis in the two cell lines. Moreover, real-time PCR and Western blotting analysis showed that ibuprofen treatment caused a reduction in survivin expression and elevation in E-cadherin expression at both mRNA and protein levels. Conclusion Ibuprofen can significantly inhibit human NSCLC proliferation and metastasis in vitro, which might be related to degreased expression of survivin and increased expression of E-cadherin.

关 键 词：布洛芬 SURVIVIN E-CADHERIN 肺肿瘤 乳腺肿瘤 

分 类 号：R73-361[医药卫生—肿瘤]