壬基酚对大鼠5-羟色胺及其2A受体的影响  

Effects of nonylphenol on 5-hydroxytryptamine and its 2A receptor in rats

作　　者：杨童旺 赖玉婷[1] 郭佑廷 欧阳俊彦[1] 林媛[1] 吕宝怡 柳春红[1,2] 

机构地区：[1]华南农业大学食品学院食品科学系,广州510642 [2]广东省食品质量安全重点实验室,广州510642

出　　处：《第二军医大学学报》2014年第12期1358-1362,共5页Academic Journal of Second Military Medical University

基　　金：广东省自然科学基金(S2011010001045);广东省科技计划项目(2012B091400001;2012B090600005)~~

摘　　要：目的观察壬基酚（NP）对大鼠血浆和尿液中5-羟色胺（5-hydroxy tryptamine,5-HT）及血小板中5-HT和5-HT2A受体含量的影响,探讨壬基酚对大鼠5-HT和5-HT2A受体影响的毒效应机制。方法将24只SD雄性大鼠分为阴性对照组和NP低、中、高剂量组[30、90、270mg/（kg·d）],隔日灌胃染毒28d后检测大鼠血浆中5-HT、血小板中5-HT和5-HT2A受体含量,并检测灌胃后收集到的24h尿液中5-HT的含量。结果染毒28d后,随NP暴露的剂量增加,各组大鼠血浆、血小板及尿液中5-HT含量升高,血小板中5-HT2A受体表达则下降。NP暴露中、高剂量组大鼠血浆及血小板中5-HT含量均高于对照组（P〈0.01;P〈0.01）,NP暴露高剂量组大鼠血小板5-HT2A受体表达低于对照组（P〈0.01）。第4~28天,NP暴露低、中、高剂量组大鼠的尿液5-HT含量均高于对照组（P〈0.01）。结论 NP暴露剂量与大鼠血小板5-HT、5-HT2A受体及血浆和尿液中5-HT含量均呈剂量-效应关系,提示NP通过影响大鼠5-HT水平和5-HT2A受体表达而产生毒效应。Objective To observe the effect of nonylphenol （NP） on plasma and urine 5-hydroxytryptamine （5-HT） levels and expression of 5-HT and of 5-HT2A receptor in the platelets of rats, so as to explore the toxic mechanisms of NP on 5-HT and 5-HT2A receptor. Methods A total of 24 male SD rats were equally randomized into 4 groups： solvent control group, NP low-dose group （30 mg/[kg·d] ）, NP medium-dose group （90 mg/[ kg·d]） and NP high-dose group （270 mg/[ kg·d]）. The animals were garaged with NP every other day for 28 days; then the plasma and 24 h urine levels of 5-HT, 5-HT and 5-HT2A expression in the platelets were examined. Results The levels of 5-HT in the plasma and platelets of rats were increased and the expression of 5-HT2A receptor in platelets was decreased with the increase of NP exposure dose. The plasma and platelet 5-HT levels in the medium- and high-NP dose groups were significantly higher than those in the control group（P〈0.01） ; the platelet level of 5-HT2A receptor in the high-NP dose group was significantly lower than that in the control group （P〈0.01）. The three NP dose groups had significantly higher urine 5-HT levels compared with the control group during 4-28 days of NP exposure（P〈0.01）. Conclusion There are dose-dependent effects between NP exposure dose and plasma, urine 5- HT levels and platelet 5-HT, 5-HT2A receptor levels in rats, suggesting that NP might exert its toxic effect by affecting 5-HT level and 5-HT2A receptor expression.

关 键 词：壬基酚 5-羟色胺 5-HT2A受体 毒效应 

分 类 号：R595.9[医药卫生—内科学]