针刺对缺氧缺血性脑损伤新生大鼠大脑皮质14-3-3蛋白表达的影响  

Effect of Acupuncture Intervention on 14-3-3 Expression in Cerebral Cortex of Hypoxic-ischemic Brain Damage Rats

作　　者：李星儿[1] 袁青[2] 唐纯志[2] 陈飞[3] 赵蓉[3] 刘龙琳 俞裕天[3] 曹勇[3] 吴家利[3] 孙铄[3] 

机构地区：[1]华南师范大学激光运动医学实验室,广州510006 [2]广州中医药大学针灸康复临床医学院,广州510405 [3]广州中医药大学,广州510405

出　　处：《针刺研究》2014年第6期466-471,共6页Acupuncture Research

基　　金：国家自然科学基金(No.81072878)

摘　　要：目的:探讨针刺对缺氧缺血性脑损伤(HIBD)新生大鼠大脑皮质中14-3-3及凋亡相关的Bcl-2家族中两大主要成员Bcl-2和Bax的影响。方法:明确预产期的SD雌性妊娠大鼠接受延迟5min剖宫产术,所得新生大鼠随机分为模型组和针刺组;接受正常剖宫产术的大鼠所产新生大鼠为假手术组;代乳雌鼠自然分娩的新生大鼠为正常组。每组8只动物。针刺组新生大鼠饲养至14d时,每天针刺"脑三针""颞三针""智三针",每个穴位刺激20s,不留针,连续7d。各组新生大鼠均于出生后21d断头取脑,用免疫组织化学染色法检测大脑皮质中14-3-3、Bcl-2和Bax蛋白的表达。结果:比较各组新生大鼠大脑皮质14-3-3的表达,假手术组和模型组显著低于正常组和针刺组(P<0.05,P<0.01);比较各组新生大鼠大脑皮质Bcl-2的表达,模型组略低于正常组(P>0.05),针刺组高于模型组(P<0.05);比较各组新生大鼠大脑皮质Bax的表达,假手术组、模型组显著高于正常组(P<0.01,P<0.05),针刺组与模型组间的差异无统计学意义(P>0.05)。结论:针刺可以提高HIBD新生大鼠大脑皮质中14-3-3和Bcl-2的表达水平。Objective To observe the effect of acupuncture therapy on 14-3-3, Bcl-2 and Bax expression levels in the cerebral cortex in neonatal rats with hypoxic-ischemic brain damage（HIBD）. Methods Timed pregnant Sprague-Dawley rat dams were delivered either vaginally （normal group）, or by C-section （sham-operation group） or by C-section with 5 min of global anoxia （anoxia group）, with 8 rats in each group. The rat pups of the anoxia group were randomly divided into model group and acupuncture group （n = 8）. Acupuncture stimulation of ＂Naosanzhen＂ ＂Niesanzhen＂ and ＂Zhisanzhen＂ acupoints was given begin- ning from the 14th day after birth, once daily for 7 consecutive days. All rat pups were killed by decapitation on day 21 after birth, and then 14-3-3, Bcl-2 and Bax immunoactivity （expression） in the cerebral cortex were detected by immunohistochemistry. Results In comparison with the normal group, the expression level of cerebral cortical 14-3-3 was significantly decreased, and that of Bax remarkably increased in the model group （P〈0.01, P〈O. 05）. Compared to the model group, cortical 14-3-3 and Bcl-2 expression levels were markedly up-regulated in the acupuncture group （P〈0.01, P〈O. 05）. Compared to the normal group, cortical 14-3-3 expression level was obviously lower, but Bax expression level significantly higher in the sham-operation group （P〈0.05, P〈O. 01）. No significant differences were found between the model and normal groups in the expression levels of Bcl-2, and between the acupuncture and model groups in the expression levels of Bax （P〉0.05）. Conclusion Acupuncture intervention can increase the expression of 14-3-3 and Bcl-2 in the cerebral cortex in HIBD rats.

关 键 词：针刺 缺氧缺血性脑损伤 宫内窘迫/宫内窒息 细胞凋亡 大脑皮质14-3-3/Bcl-2/Bax 

分 类 号：R245.311[医药卫生—针灸推拿学]