大鼠血清网膜素-1水平与胰岛素抵抗的相关性  

Correlation of serum omentin-1 level and insulin resistance in rats

作　　者：王楠楠[1] 赵晓娟[1] 陈薇[1] 白淑亭[1] 杨博文[2] 

机构地区：[1]中国医科大学附属第一医院内分泌科,辽宁沈阳110001 [2]中国医科大学附属第一医院流行病科,辽宁沈阳110001

出　　处：《中国病理生理杂志》2014年第12期2276-2279,共4页Chinese Journal of Pathophysiology

基　　金：辽宁省科学技术计划资助项目(No.2009225010-10)

摘　　要：目的:建立胰岛素抵抗大鼠模型,探讨血清网膜素-1水平与胰岛素抵抗的相关性。方法:30只SPF级雄性Wistar大鼠随机分为正常对照组(NC,n=15)、高脂饮食组(HF,n=15),自由摄食、饮水,NC组给予普通饲料,HF组给予高脂饲料诱导胰岛素抵抗模型。10周后2组各取5只行高胰岛素正葡萄糖钳夹实验评估模型,ELISA法检测血清网膜素-1,放射免疫法检测血清基础胰岛素。结果:(1)各组大鼠基础血糖比较无显著差异(P>0.05)。(2)HF组血清基础胰岛素水平较NC组明显升高(P<0.05),但血清网膜素-1水平较NC组明显降低(P<0.01)。(3)Pearson相关性分析示血清网膜素-1分别与胰岛素(r=-0.654,P<0.01)和游离脂肪酸(r=-0.446,P<0.05)呈负相关。结论:在大鼠胰岛素抵抗阶段,血清网膜素-1水平已经开始降低;基础胰岛素水平随着血清网膜素-1水平的降低而升高,推测血清网膜素-1水平降低可能成为胰岛素抵抗、糖尿病及心血管疾病的早期提示因子。AIM： To establish the insulin resistance rat model for evaluating the correlation of omentin-1 level and insulin resistance. METHODS ： SPF male Wistar rats （ n = 30） were randomly divided into normal control group （NC, n = 15） and high-fat diet group （HF, n = 15）. The rats in NC group were fed with basic diet. The insulin resistant model was established by feeding the rats with high-fat diet in HF group. After 10 weeks, 5 rats in each group were as- sessed by the technique of hyperinsulinaemic-euglycaemic clamp. After the insulin resistant model was successfully estab- lished, the body weight and fasting blood glucose were detected. The concentration of fasting serum omentin-1 was analyzed by ELISA. Fasting serum insulin was measured by radioimmunoassay. RESULTS： No difference of fasting blood glucose between the 2 groups was observed. The level of fasting serum insulin in HF group was significantly higher than that in NC group （P 〈 0. 05 ）. The level of serum omentin-1 in HF group were significantly decreased compared with NC group （P 〈 0. 01 ）. Pearson＇s correlation analysis showed that negative correlations between serum omentin-1 and fasting serum insulin （ r = - 0. 654, P 〈 0. 01 ）, serum omentin-1 and free fatty acid （ r = - 0. 446, P 〈 0. 05 ） was found. CONCLU- SION： In rats, serum omentin-1 level began to decrease at insulin resistance stage. As serum omentin-1 level decreased, the basal insulin level increased, indicating that decreased serum omentin-1 level may be an early factor of IR, diabetes and cardiovascular diseases.

关 键 词：胰岛素抵抗 高胰岛素正葡萄糖钳夹 网膜素-1 

分 类 号：R587[医药卫生—内分泌]