KK-42对日本沼虾D_3期头胸甲表皮结构的影响  

Effect of KK-42 on the carapace structure in Macrobrachium nipponense during premolt D_3 stage

作　　者：吕艳杰[1] 陈香丽[1] 郭爱莲[1] 王佩[1] 宁黔冀[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《水产学报》2014年第12期1964-1969,共6页Journal of Fisheries of China

基　　金：国家自然科学基金(30940008);河南省基础与前沿技术研究项目(142300410021)

摘　　要：为探讨KK-42缩短日本沼虾幼虾蜕皮周期的机制,实验采用组织学方法观察了幼虾头胸甲表皮结构,定量测定了肝胰腺N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)的活力。将体长(3.3±0.5)cm的日本沼虾随机分为两组,分别用1.95×10-4mol/L的KK-42溶液(实验组)或不含KK-42的溶液(对照组)浸泡处理1 min之后,选取处于蜕皮前期D3期的日本沼虾,观察头胸甲表皮结构,测定肝胰腺NAGase活力。结果显示,KK-42处理后第1天,头胸甲外表皮厚度与对照组相比显著增长42.83%;第3天和第9天,内表皮的厚度同比提高70.72%、77.27%,整个头胸甲的厚度同比显著增厚。KK-42处理后第3天,上皮细胞的高度比对照组提高31.69%。KK-42处理后6、12和24 h,NAGase活力分别比相应的对照组升高42.53%、34.28%和18.36%。研究表明,KK-42处理可显著增加D3期日本沼虾头胸甲外骨骼的厚度,提高肝胰腺NAGase活力。In order to research the possible mechanism that the duration of molting cycle of the juvenile prawn Macrobrachium nipponense was shortened by KK-42, the cuticle structure of carapace was investigated by the histological method and the N-acetyl-13-D-glucosaminidase （NAGase）activity from hepatopancreas was quantitatively analyzed using ultraviolet absorption method before and after KK-42 treatment. The prawns with body length of （3.3 ± 0.5 ） cm were randomly divided into two groups ： KK-42 treatment group and control one. The prawns were soaked for 1 min in KK-42 solution at a concentration of 1.95× 10-4 mol/L （ KK-42 treatment group） or 0 mol/L （ control group）, respectively. Afterwards, the carapaces derived from late premolt （ D3 ） prawns were chosen to be used for histological observation and thickness measurement of layered exoskeleton, meanwhile, the hepatopancreas NAGase activity in stage D3 M. nipponense was quantitatively analyzed. The results showed that the exocuticle thickness of carapace significantly increased by 42. 83% compared with the corresponding control on 1st day after KK-42 treatment, and the endocuticle thickness as well as the whole exoskeleton thickness significantly increased by 70.72% （P 〈 0.01 ） ,77.27% （P 〈 0.01） and 38.69% （P 〈 0.01 ） ,52.23% （P 〈 0.01 ）, respectively, compared to the corresponding control on the 3rd day and the 9th day after KK-42 treatment. A 31.69% rise in the height of epithelial cells was obtained on the 3rd day after KK-42 treatment compared with the corresponding control. The NAGase activity significantly increased by 42.53% （P 〈 0.01 ）,34.28% （P 〈 0.01 ）和 18.36% （P 〈 0.05 ）, respectively, at 6 h, 12 h and 24 h after KK-42 administration compared to the corresponding control. The results reveal that KK-42 treatment can significantly increase the cuticle thickness of carapace,and promote NAGase activity from hepatopancreas.

关 键 词：日本沼虾 KK-42 头胸甲 NAGASE 

分 类 号：Q174[生物学—水生生物学] S968.2[生物学—普通生物学]