干露对三疣梭子蟹抗氧化和应激能力的影响  

Effects of air-exposure stress on antioxidant capacity and stress response of swimming crab( Portunus trituberculatus)

作　　者：姜娜[1] 王芳[1] 路允良[1] 董双林[1] 

机构地区：[1]中国海洋大学水产学院海水养殖教育部重点实验室,山东青岛266003

出　　处：《水产学报》2014年第12期1996-2004,共9页Journal of Fisheries of China

基　　金：国家"十二五"科技支撑计划(2011BAD13B03)

摘　　要：在实验室条件下,研究了干露时间（0.5、1.5和3 h）胁迫对三疣梭子蟹抗氧化能力和应激能力的影响,初步探讨了能够较灵敏地指示梭子蟹健康状况的胁迫指标。结果显示：（1）干露时间胁迫对超氧化物歧化酶（SOD）活力和丙二醛（MDA）含量均无显著影响（P〉0.05）;但干露时间显著影响肝胰脏总抗氧化能力（T-AOC）水平、热休克蛋白70（HSP70）表达量和肌肉乳酸含量（P〈0.05）。（2）0.5 h干露胁迫后,SOD活力在入水恢复4 h时达到峰值;MDA含量在入水恢复2-10 h内显著高于对照组水平（P〈0.05）;T-AOC水平在恢复阶段基本维持在对照组水平。1.5和3 h干露后,SOD活力在入水恢复阶段一直维持在对照组水平,且各时间点与对照组差异不显著（P〉0.05）。1.5 h干露后MDA含量在入水恢复阶段呈先升高后降低趋势,各时间点与对照组差异不显著（P〉0.05）;而3 h干露后入水恢复10 h时显著增加（P〈0.05）。1.5 h干露后入水恢复4 h时,T-AOC水平显著高于对照组（P〈0.05）;3 h干露后入水恢复0.5 h时显著升高（P〈0.05）,2-10 h内基本恢复到对照组水平。干露时间胁迫后,乳酸含量均在入水恢复0.5 h时达到最大值;HSP70表达量均在入水恢复2-4 h内达到峰值。研究表明,相比于其他所选指标,T-AOC水平能够较敏感地反映干露时间胁迫对机体的生理状态的影响;SOD和MDA相互配合能够较灵敏地反映不同干露时长后机体在恢复阶段的免疫水平。The purpose of this study was to evaluate the stress indicators which can reflect the physiology of swimming crabs（Portunus trituberculatus）sensitively during air-exposure and subsequent recovery. The effects of 0.5,1.5 and 3 h air-exposure stress on antioxidant capacity and stress response of crabs with an average weight of （87.93 ± 1.24）g were investigated by determining hepatopancreas superoxide dismutases （SOD）activity, total antioxidant capacity （ T-AOC ）, malondialdehyde （ MDA ） and heat shock protein 70 （ HSP70 ） level and muscles lactate contents. Crabs were sampled at 0.5,1.5 and 3 h during exposure to air,and at 0,2,4 and 10 h during following recovery, with crabs in seawater of （22.5± 0.5 ） ℃ as control. The main results showed that： （ 1 ） No significant effects of air exposure stress on SOD activity and MDA content in hepatopancreas were observed （P 〉 0.05）. However, air exposure stress had a significant influence on the T-AOC level, the content of HSP70 in hepatopancreas and muscles lactate contents. （2） After 0.5 h air-exposure stress, the SOD activity reached the maximum value when crabs had recovered for 4 h. During the recovery from 2 h to 10 h after 0.5 h stress, the MDA contents were significantly higher than that of control （P 〈 0.05 ）. No significant differences of the T-AOC level in hepatopancreas were observed during recovery after 0.5 h of air exposure （P 〉 0.05）. The SOD activity was similar to control after 1.5 h and 3 h of air exposure stress,and there were no significant differences between control and time intervals （ P 〉 0.05 ）. The MDA content of crabs exposed to air for 1.5 h increased first and then decreased during recovery, with no significant differences observed between control and time intervals （P 〉 0.05）. However,after 3 h of air exposure ,the MDA content significantly increased at 10 h during recovery （P 〈 0.05）. Besides ,the T-AOC level reached the maximum value at 4 h after 1.5 h of air exposur

关 键 词：三疣梭子蟹 干露时间胁迫 入水恢复 抗氧化 应激 

分 类 号：Q178.1[生物学—水生生物学] S968.2[生物学—普通生物学]