p53转染黑色素瘤细胞修饰的树突状细胞疫苗体外抗肿瘤作用的研究  

Study of Anti-tumor Immunity In Vitro Induced by DC Vaccines Loaded by p53-modified Tumor Antigens

作　　者：王冰[1,2] 王红梅[1] 刘燕玲[1] 周晓燕[1] 周慧容 徐方云[1] 闵卫平 

机构地区：[1]南昌大学医学院病理生理学教研室,南昌330006 [2]浙江省立同德医院,杭州310012 [3]江西省医学科学研究院,南昌330006

出　　处：《中国细胞生物学学报》2014年第12期1616-1621,共6页Chinese Journal of Cell Biology

基　　金：江西省科技支撑计划(批准号:2009BSD09600)资助的课题~~

摘　　要：利用野生型p53质粒转染黑色素瘤B16细胞,反复冻融法提取p53修饰的肿瘤抗原(p53-Ag),将抗原体外冲击同基因小鼠骨髓来源的树突状细胞(dendritic cells,DC)制备特异性DC肿瘤疫苗;观察DC诱导的淋巴细胞增殖反应和细胞毒性T淋巴细胞(cytotoxic T lymphocytes,CTL)对黑色素瘤细胞的细胞毒效应,分析其诱导肿瘤抗原特异性免疫应答的机制。结果显示,p53-肿瘤抗原冲击的DC可显著刺激淋巴细胞增殖,其诱导的CTL效应对肿瘤细胞也有很好的杀伤效果。Tumor antigen （p53-Ag） was extracted from mouse B16 melanoma cells transfected with the wild-p53 plasmid by freeze-thaw method. Then it was loaded onto dendritic cells （DC） harvested from the bone marrow to obtain DC tumor vaccine. The antigen-specific killing effect of cytotoxic T lymphocytes （CTL） activated by DC vaccine on cancer cells in vitro and the ability to induce proliferation of T cells were detected by MTT assay. The experiment results showed that p53-modified DC vaccine significantly up-regulated the proliferation of T lym- phocytes and the killing rate by CTL on cancer cells.

关 键 词：树突状细胞 P53 肿瘤抗原 疫苗 

分 类 号：R739.5[医药卫生—肿瘤]