三七总皂苷对支气管哮喘小鼠气道炎症及气道重建的影响  

The Effects of Panax Notoginseng Saponins on Airway Inflammation and Remodeling in a Mouse Model of Bronchial Asthma

作　　者：聂修鹏 毛小琴[2] 李丰良[2] 彭静[3] 牛华[2] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]云南省第一人民医院检验科,云南昆明650032 [3]云南中医学院病原生物学与免疫学实验室,云南昆明650032

出　　处：《现代生物医学进展》2015年第1期39-44,共6页Progress in Modern Biomedicine

基　　金：云南省应用基础研究计划项目(2011FB217;2011FB216)

摘　　要：目的:探讨三七总皂苷对支气管哮喘小鼠气道炎症及气道重建的影响。方法:选择昆明种小鼠24只,将其随机分为三七总皂苷(PNS)治疗组、阳性对照组(哮喘组)和阴性对照组,每组8只。小鼠腹腔内注射氢氧化铝-卵清蛋白悬浊液(OVA-Al(OH)3)进行致敏,致敏完成后用2%卵清蛋白溶液(OVA)雾化激发,在激发前30分钟给药治疗,雾化激发6周。建模完成后,做心肺组织灌流,取左肺固定做H&E染色,镜下观察评估气道病理学改变(气道狭窄阻塞率、气道上皮坏死糜烂率、上皮细胞杯状化生率、炎性细胞浸润率及平滑肌增生率);行免疫组织化学染色,用图像分析软件半定量测量转化生长因子-beta1(TGF-β1)的含量;行特殊染色,用图像分析软件测量基底膜厚度;取右肺组织,利用酶联免疫吸附法(ELISA)测定肺组织匀浆白介素-17(IL-17)因子的水平。结果:1各组小鼠经心肺组织灌流后,与哮喘组比较,三七治疗组小鼠的肺部红肿减轻,肺表颜色较正常组略微泛红。2三七治疗组气道狭窄率、上皮细胞坏死率、炎细胞浸润率、平滑肌增生率均显著低于哮喘组(P<0.05),但与正常组比较无显著性差异(P>0.05)。3三七治疗组气道上皮细胞TGF-β1含量显著低于哮喘组(P<0.001),肺组织白介素-17含量降低至哮喘组一半以下(P>0.05),与对照组相比均无显著性差异(P=0.94,P=0.23)。4哮喘组、三七组和对照组小鼠的支气管基底膜厚度分别为(0.7893±0.014)、(0.7216±0.016)、(0.5655±0.012)μm,哮喘组显著高于三七组和对照组,均具有显著性差异(P<0.05)。结论:三七总皂苷可以有效改善支气管哮喘小鼠的气道重建,并抑制其气道炎性。Objective： To explore the effects of Panax notoginseng saponins on the airway inflammation and remodeling in a mouse model of bronchial asthma. Methods： Twenty-four Kunming mice were selected and randomly divided into Panax notoginseng saponins treatment（PNS） group, positive control group（asthma group） and control group. Each group was fed eight mice. Mice were sensitized by intraperitoneal（i.p.） injections of OVA-AL（OH）3suspension and exposed to 2% OVA solution for challenge after sensitization. 0.5 h before challenge for 6 weeks. After the completion of modeling, the cardiopulmonary tissues were removed for tissue perfusion. The left lung was removed for stainning by hematoxylin and eosin（HE） to assess airway pathological alteration under a light microscopy（the ratio of airway stenosis, the ratio of erosion and necrosis of airway epithelial cells, the ratio of goblet cell metaplasia, the ratio of inflammatory cell infiltration and the ratio of smooth muscle hyperplasia）. The levels of TGF-β1 was detected by immunohistochemical staining, and analyzed using a Leica imaging system and computerized image analysis software to semi-quantified. The levels of IL-17 in right lung tissue supernatants were measured by ELISA. Results： 1After cardiopulmonary perfusion, in PNS group, the red and swollen in lung tissue was alleviated in comparison with the asthma group, and a little red in comparison with the control group. 2In PNS group, the ratio of airway stenosis, the ratio of erosion and necrosis of airway epithelial cells, the ratio of goblet cell metaplasia, the ratio of inflammatory cell infiltration and the ratio of smooth muscle hyperplasia were significantly lower than those of the asthma group（P〈0.05）. But no significant difference was observed between PNS group and control group（P〈0.05）. 3The expression of TGF-β1 in PNS group was significantly lower than that of the asthma group（P〈0.001）. The level of IL-17 in PNS group decreased to less than a half of asthma grou

关 键 词：支气管哮喘 转化生长因子 白介素-17 基底膜厚度 

分 类 号：Q95-3[生物学—动物学] R562.25[医药卫生—呼吸系统]