高压氧治疗对大鼠术后认知功能障碍的影响  

Effect of hyperbaric oxygen treatment on postoperative cognitive dysfunction in rats

作　　者：赵柏松 田悦[2] 谭永红 宋兴荣 

机构地区：[1]广州市妇女儿童医疗中心麻醉科,510623 [2]中国医科大学附属盛京医院麻醉科

出　　处：《中华麻醉学杂志》2014年第12期1442-1445,共4页Chinese Journal of Anesthesiology

基　　金：贝朗麻醉科学研究基金（BBF2012-012）

摘　　要：目的 评价高压氧治疗对大鼠术后认知功能障碍的影响.方法 雄性SD大鼠18只,体重260-290 g,应用脑立体定位仪于大鼠海马区注射Aβ140淀粉样蛋白制备大鼠术后认知功能障碍模型.采用随机数字表法,将其分为3组（n=6）：生理盐水组（NS组）海马区注射0.9％生理盐水2出;术后认知功能障碍组（POCD组）海马区注射Aβ1.淀粉样蛋白2μl;高压氧治疗组（HBO组）海马区注射Aβ1.淀粉样蛋白2ul,并于术后1-5d行高压氧治疗60 min,1次/d.于术后7、14和21 d时行水迷宫实验,记录游泳距离、速度和逃避潜伏期.于术后21 d水迷宫实验结束后处死大鼠,取海马组织,采用免疫组化法测定海马星形胶质细胞活化情况,计算星形胶质细胞活化率,采用ELISA法测定海马TNF-α含量.结果 术后7和14 d时,3组间各项水迷宫行为学指标比较差异无统计学意义（P＞0.05）.术后21、d时,与NS组相比,POCD组大鼠游泳距离和逃避潜伏期延长,海马星形胶质细胞活化率升高,TNF-α含量增多,HBO组大鼠游泳距离和逃避潜伏期延长,海马星形胶质细胞活化率升高（P＜0.05）,海马TNF-α含量差异无统计学意义（P＞0.05）;与POCD组相比,HBO组大鼠游泳距离和逃避潜伏期缩短,海马星形胶质细胞活化率降低,TNF-α含量减少（P＜0.05）.3组各时点大鼠游泳速度比较差异无统计学意义（P＞0.05）.结论 高压氧治疗可有效缓解大鼠术后认知功能障碍,其机制与高压氧抑制海马星形胶质细胞活化及炎性反应有关.Objective To evaluate the effect of hyperbaric oxygen （HBO） treatment on postoperative cognitive dysfunction （POCD） in rats.Methods Eighteen healthy male Sprague-Dawley rats,weighing 260-290 g,were anesthetized with intraperitoneal 10％ chloral hydrate 350 mg/kg.POCD was induced by injecting Aβ-40 2μl into the bilateral hippocampi by using a brain stereotaxic apparatus.The rats were randomly divided into 3 groups （n =6 each） using a random number table：normal sahne group （group NS）,POCD group,and HBO treatment group（groupHBO）.0.9％ normal saline 2 μl was injected into hippocampus in group NS.In group POCD,Aβ0 2 μl was injected into hippocampus.In group HBO,Aβ 2μl was injected into hippocampus,and then the rats received hyperbaric oxygen treatment lasting for 60 min once a day within 1-5 days after operation.Morris water maze test was performed on 7,14 and 21 days after operation in each group and the swimming distance and speed and escape latency were recorded.The animals were sacrificed after the end of test,the hippocampi were then removed to detect the activation of astrocytes （by immuno-histochemistry） and content of tumor necrosis factor-α （TNF-α） （by ELISA）.Resets There were no significant differences in the parameters of behavior in Morris water maze test on 7 and 14 days after operation between the three groups.Compared with group NS,the swimming distance and escape latency were significantly prolonged,and the activation of astrocytes and TNF-α content were increased on 21 days after operation in group POCD,and the swimming distance and escape latency were significantly prolonged,the activation of astrocytes was increased,and no significant change was found in TNF-α content on 21 days after operation in group HBO.Compared with group POCD,the swimming distance and escape latency were significantly shortened,and the activation of astrocytes and TNF-α content were decreased in group HBO.There was no significant difference in the swimming speed at each time poin

关 键 词：高压氧 认知障碍 

分 类 号：R614[医药卫生—麻醉学]