肺动脉高压大鼠肺小动脉增殖内膜缺氧诱导因子-1α表达的变化  

Changes m expression of hypoxia-inducible factor 1alpha in proliferated intima of pulmonary arterioles in rats with pulmonary hypertension

作　　者：孙青[1,2] 申艳[3] 颜洁[4] 李冰冰[3] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,221004 [2]江苏省麻醉与镇痛应用技术重点实验室 [3]南京大学医学院附属鼓楼医院麻醉科,210008 [4]南京医科大学附属妇幼保健院麻醉科

出　　处：《中华麻醉学杂志》2014年第12期1471-1474,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金青年科学基金（30901406/C160203）;江苏省自然科学基金面上项目（BK2012532）;南京青年卫生人才项目

摘　　要：目的 评价肺动脉高压大鼠肺小动脉增殖内膜缺氧诱导因子-1α （HIF-1α）表达的变化.方法 成年雄性SD大鼠32只,2月龄,体重200 - 230 g,采用随机数字表法,将其分为4组（n=8）：假手术组（S组）、左肺切除术组（P组）、野百合碱组（M组）和野百合碱联合左肺切除术组（PM组）.M组和PM组于术后7d皮下注射野百合碱60 mg/kg.于术后35 d测定平均肺动脉压（mPAP）和右室收缩压（RVSP）,并计算右心室肥厚指数（RVHI）、肺小动脉中膜厚度（MT）百分比,进行血管阻塞评分（VOS）,并测定肺小动脉HIF-1α、α-平滑肌肌动蛋白（α-SMA）和增殖细胞核抗原（PCNA）的表达.结果 与S组比较,P组MT百分比升高,肺小动脉α-SMA表达上调,M组mPAP、RVSP、RVHI和MT百分比升高,PM组mPAP、RVSP、RVHI、MT百分比和VOS评分升高,M组和PM组肺小动脉HIF-1α、α-SMA和PCNA表达上调（P＜0.05）;与p组比较,M组和PM组mPAP、RVSP、RVHI、MT百分比升高,肺小动脉HIF-1α、α-SMA和PCNA表达上调（P＜0.05）;与M组比较,PM组RVSP、RVHI、MT百分比和VOS评分升高,肺小动脉HIF-1α、α-SMA和PCNA表达上调（P＜0.05）.结论 肺动脉高压大鼠肺小动脉内膜增殖的机制与HIF-1α表达上调,促进血管平滑肌细胞增殖有关.Objective To evaluate the changes in the expression of hypoxia-inducible factor 1 alpha （HIF-1α） in the proliferated intima of pulmonary arterioles in rats with pulmonary hypertension.Methods Thirty-two pathogen-free male Sprague-Dawley rats,aged 2 months,weighing 200-230 g,were used in the study.Thirty-two rats were randomly divided into 4 groups with 8 animals in each group using a random number table：shamoperation group （group S）,left pneumonectomy group （group P）,monocrotaline group （group M）,and left pneumonectomy combined with monocrotaline group （group PM）.The rats underwent left pneumonectomy in P and PM groups.In M and PM groups,monocrotaline 60 mg/kg was injected subcutaneously at 7 days after operation.On day 35 after operation,mean pulmonary artery pressure （mPAP） and right ventricle systolic pressure （RVSP）were measured via direct puncture of right ventricle outflow tract with gauge 24 iv catheter connected to pressure transducer.At the end of experiment,the hearts and right lobes of the lung were excised for measurement of right ventricle hypertrophy index （RVHI） and the thickness of the medial layer （tunica media） of pulmonary arterioles （MT）.Vascular occlusion score （VOS） was performed.The expression of HIF-1α,α-smooth muscle actin （α-SMA）,and proliferating cell nuclear antigen （PCNA） in the pulmonary arterioles was determined.Results Compared with S group,MT was significandy increased,and the expression of α-SMA was up-regulated in P group,mPAP,RVSP,RVHI and MT were increased in M group,mPAP,RVSP,RVHI,MT and VOS were increased in PM group,and the expression of HIF-1α,α-SMA,and PCNA was up-regulated in M and PM groups.Compared with P group,mPAP,RVSP,RVHI and MT were significantly increased,and the expression of HIF1α,α-SMA,and PCNA was up-regulated in M and PM groups.Compared with M group,RVSP,RVHI,MT and VOS were si gnificantly increased,and the expression of HIF-1α,α-SMA,and PCNA was up-regulated in PM group.Conclusion The mechanis

关 键 词：高血压 肺性 缺氧诱导因子1 Α亚基 肺动脉 细胞增殖 

分 类 号：R543.2[医药卫生—心血管疾病]