烟碱或迷走神经电刺激对大鼠移植肺损伤的影响  

Effects of nicotine or electric vagal stimulation on injury to the transplanted lungs in rats

作　　者：丁文刚[1] 刘天华[1] 王琦[1] 李文志[1] 

机构地区：[1]哈尔滨医科大学附属第二医院麻醉科,150086

出　　处：《中华麻醉学杂志》2014年第12期1475-1477,共3页Chinese Journal of Anesthesiology

基　　金：黑龙江省自然科学基金（D200840）;黑龙江省教育厅基金（11541202）

摘　　要：目的 评价烟碱或迷走神经电刺激对大鼠移植肺损伤的影响.方法 雄性Wistar大鼠,体重250 - 300 g,制备大鼠异体同位左肺移植模型.取肺移植成功的大鼠24只,采用随机数字表法,将其分为3组（n=8）：对照组（C组）、烟碱组（NI组）和迷走神经电刺激组（VS组）.NI组于肺移植术后15 min时腹腔注射烟碱2 mg/kg; VS组于肺移植术后15 min时电刺激右侧迷走神经干（5V,2 ms,l Hz）20 min,间隔10 min,共2次.于肺移植术后15、30、45、75、105和135 min时,采集动脉血样进行血气分析;在肺移植术后135 min时取动脉血样,采用ELISA法测定血浆IL-6、IL-8和TNF-α的浓度.并 取移植肺组织,光镜下行肺损伤评分（LIS）,测定肺组织湿重.干重（W/D）比值.结果 与C组比较,NI组和VS组PaO2、pH值升高,肺组织LIS、W/D比值、血浆IL-6、IL-8和TNF-α的浓度降低（P＜0.05）.结论 烟碱或迷走神经电刺激均可减轻大鼠移植肺损伤.Objective To evaluate the effects of nicotine or electric vagal stimulation on injury to transplanted lungs in rats.Methods Twenty-four male Wistar rats with transplanted lung,weighing 250-300 g,were randomly divided into 3 groups （n =8 each） using a random number table：control group （group C）,nicotine group （group NI） and electric vagal stimulation group （group VS）.Nicotine 2 mg/kgwas injected intraperitoneally at 15 min after lung transplantation in group NI.Right cervical vagus nerve trank was stimulated for 20 min with continuous electric rectangular pulses （5 V,2 ms,1 Hz） at 10 min interval twice in total starting from 15 min after lung transplantation in group VS.Arterial blood samples were then collected for blood gas analysis at 15,30,45,75,105 and 135 min after lung transplantation.Arterial blood samples were then collected at 135 min after lung transplantation for determination of the plasma concentration of interleukin-6 （IL-6）,IL-8 and tumor necrosis factor-α （TNF-α） by ELISA.The rats were sacrificed at 135 min after lung transplantation and the tissue of transplanted lung was removed for determination of lung injury score （LIS） according to the pathological changes obtained with light microscope,and of wet/dry lung weight ratio （W/D ratio）.Results Compared with group C,PaO2 and pH value were significantly increased,and LIS,W/D ratio,and the concentrations of plasma IL-6,IL-8 and TNF-α were decreased in NI and VS groups.Conclusion Nicotine or electric vagal stimulation can attenuate injury to transplanted lungs in rats.

关 键 词：肺移植 迷走神经 电刺激 胆碱能激动剂 

分 类 号：R655.3[医药卫生—外科学]