山东灰霉病菌对腐霉利的抗药性检测  

Resistance Detection for Botrytis cinerea Against Procymidone in Shandong Province

作　　者：刘超[1,2] 张悦丽[2] 张博[2] 李长松[2] 石磊[1] 齐军山[2] 

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273165 [2]山东省农业科学院植物保护研究所/山东省植物病毒学重点实验室,济南250100

出　　处：《农学学报》2014年第12期30-32,103,共4页Journal of Agriculture

基　　金：公益性行业(农业)科研专项经费"保护地果蔬灰霉病绿色防控技术研究与示范"(201303025);山东省现代农业产业技术体系

摘　　要：为监测灰霉病菌对腐霉利的抗药性，2012年12月到2013年5月，在山东省内10个市县，采集灰霉病菌样本150余份，分离得到灰葡萄孢（Botrytis cinerea）菌株130株，采用生长速率法对130株灰霉病菌进行抗药性检测。结果表明，腐霉利对灰霉病菌的EC50的范围为0.1208-12.486μg/mL，平均EC50为4.03μg/mL，抗性频率达到67.69%。其中低抗菌株占52.38%，中抗菌株占19.048%，未检测到高抗菌株。本研究可为灰霉病的防治特别是杀菌剂的轮换使用提供依据。In order to monitor procymidone resistance of Botrytis cinerea, from December of 2012 to May of 2013, many Botrytiscinereasamples were collected from 10 cities in Shandong, and 130 isolates were purified from them. The resistances of Botrytis cinerea were determined in 130 isolates by measuring the mycelia growth on the fungicide-amended media. The means of the 50% effective concentration values （EC50） were 4.03 μg/mL. The resistance frequency was 67.69%, 52.38% of Botrytis cinerea was low resistant strains, 19.048% of Botrytis cinerea was moderate resistant strains; there was no high resistant strain. The results provided a theoretical basis for using fungicides efficiently to prevent and control botrytiscinereain the field.

关 键 词：灰葡萄孢 杀菌剂 腐霉利 抗药性 

分 类 号：S482.2[农业科学—农药学]