OPA柱前衍生HPLC法测定瓜蒌皮中精氨酸的含量  

HPLC Determination of Arginine in Trichosanthes Pericarpium with OPA by Pre-column Derivatization

作　　者：郝变 潘丽丽[1] 袁少雄[1] 杨颂[1] 李亭亭[1] 徐何方 李向日[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中医药学报》2014年第6期17-19,共3页Acta Chinese Medicine and Pharmacology

基　　金：保健食品中违禁物质检测技术研究(2012BAK08B02)

摘　　要：目的：建立柱前衍生HPLC法测定瓜蒌皮中精氨酸含量的方法。方法：采用邻苯二甲醛（OPA）为衍生化试剂,色谱柱为Eclipse XDB C18（4.6mm×250mm,5μm）;流动相A为甲醇-乙腈-水（45∶45∶10）,流动相B为0.01mol/L磷酸氢二钠-0.01mol/L硼酸钠的缓冲溶液（p H=8.2）,梯度洗脱,流速为1m L/min;检测波长为338nm。结果：该方法可以对瓜蒌皮中精氨酸含量准确定量,其浓度在12-4 000mg/L范围内,线性关系良好（R^2=0.999 9）。方法精密度、重复性均小于2%,平均加样回收率为102.36%,RSD为1.89%（n=5）。10批不同产地瓜蒌皮中精氨酸测定结果差异较大,其中,山东（道地产区）瓜蒌皮中精氨酸含量最多。结论：该方法衍生方便、快速,产物单一,测定结果准确、可靠,可用于瓜蒌皮中精氨酸的含量测定。Objective： To establish a pre-column derivatization method of HPLC to determine the constituent of arginine in Trichosanthes pericarpium. Methods： Orthophthalaldehyde（OPA） was used as a derivatization reagent. Chromatographic condition： Eclipse XDB C18 HPLC column,（4. 6mm × 250 mm,5μm）,mobile phase： gradient elution of methanol-acetonitrile-water（45∶ 45∶ 10）（A） and disodium hydrogen phosphate-sodium borate buffer（ca. 0. 01 mol / L,p H = 8. 2）（B）,column temperature： 30℃,detector wavelength： 338 nm. Results： The method was simple and accurate with good reproducibility and could be used for the quantitative analysis of arginine in Trichosanthes pericarpium. It had a good linear relationship in the scope of 12 - 4 000 mg / L（R^2= 0. 999 9）. The RSD of the method in precision and repeatability was less than 2%. The average recovery was 102. 36%,RSD was 1. 89%（n = 5）. The contents of arginine in 10 batches of Trichosanthes pericarpium were different,among which the content of arginine in Shandong（real estate areas） was the highest. Conclusion： The method is accurate,reliable and sensitive,which can be used for the quantitative analysis of arginine in Trichosanthes pericarpium.

关 键 词：邻苯二甲醛 瓜蒌皮 精氨酸 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]