早期肠内营养促进烧伤后肠道增殖修复的实验研究  

Effect of early enteral nutrition on the repair of intestinal proliferation after burn

作　　者：王逸娟 刘曼曼[1] 徐淑秀[1] 吴丹[2] 吴炜[2] 彭曦[2] 

机构地区：[1]蚌埠医学院护理学系,安徽蚌埠233030 [2]第三军医大学西南医院全军烧伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038

出　　处：《蚌埠医学院学报》2014年第12期1607-1610,1614,共5页Journal of Bengbu Medical College

基　　金：重庆市自然科学基金重点资助项目(cstc2013jj B0140)

摘　　要：目的：观察早期肠内营养对烧伤大鼠回肠黏膜增殖修复的影响并探讨其机制。方法：采用30%体表面积Ⅲ度烧伤大鼠模型,将88只Wistar大鼠随机分为假烧伤对照组（ C组）、早期肠内营养组（ EEN组,烧伤后6 h开始实施肠内营养）和延迟肠内营养组（DEN组,烧伤后24 h开始实施肠内营养）。观察伤烧前（PBD 0）及烧伤后0.5 d（PBD 0.5）、1 d（PBD 1）、3 d（PBD 3）、7 d（PBD 7）和10 d（PBD 10）大鼠回肠黏膜3H-胸腺嘧啶核苷（3H-TdR）、3H-尿嘧啶核苷（3H-Uridine）和3H-亮氨酸（3 H-Leucine）掺入率、增殖指数及回肠黏膜DNA、RNA和蛋白质含量的变化。结果：烧伤后大鼠回肠黏膜3 H-TdR、3 H-Uridine掺入率各时点均明显低于烧伤前,PBD1-PBD10 EEN组3 H-TdR、3 H-Uridine、3 H-Leucine掺入率均较DEN组增高（ P〈0.05-P〈0.01）。烧伤后大鼠回肠黏膜增殖指数均低于烧伤前 PBD0,EEN 组在 PBD1-PBD10均高于 DEN 组（P 〈0.05-P 〈0.01）。大鼠回肠黏膜DNA、RNA和蛋白质含量PBD1-PBD10均显著低于PBD0,PBD3-PBD10 EEN组DNA、RNA和蛋白质含量均较DEN组增高（P〈0.05-P〈0.01）。结论：早期肠内营养有效地降低了烧伤后肠黏膜增殖受抑程度,促进了肠黏膜修复。Objective：To explore the effects of early enteral nutrition on the repair of intestinal proliferation after burn in rat,and its mechanism. Methods：Eighty-eight Wistar rats with 30% of body surface area Ⅲ degree burn were randomly divided into the normal control group（C group）,early enteral nutrition（EEN group） and delayed enteral nutrition（DEN group）. The EEN group and DEN group were treated with enteral nutrition at 6 and 24 hours after burn,respectively. The incorporation rates and proliferation indexes of 3 H-thymidine nucleoside 3 H-TdR,3 H-Uridine and 3 H-Leucine, and the levels of DNA, RNA and protein in intestinal mucosal were detected before burn and at 0. 5,1,3,7 and 10 days after burn. Results：The incorporation rates of 3 H-TdR and 3 H-Uridine in ileum mucosal of rats at each points after burn were significantly lower than those before burn. The incorporation rates of 3 H-TdR,3 H-Uridine and 3H-Leucine at 1 to 10 days after burn in EEN group were higher than those in DEN group（P〈0. 05 to P〈0. 01）. The proliferation indexes in intestinal mucosal of rats after burn were lower than those before burn,the incorporation rates at 1 to 10 days after burn in EEN group were higher than that in DEN group（P〈0. 05 to P〈0. 01）. The levels of DNA,RNA and protein of rat ileum mucosal all group decreased remarkably after burn. The levels of DNA,RNA and protein in EEN group were obviously higher than those in DEN group （P〈0. 05 to P 〈0. 01）. Conclusions：The early enteral nutrition can effectively reduce the inhibition of intestinal mucosal proliferation,and promote the repair of intestinal mucosa after burn.

关 键 词：肠内营养 肠道 增殖 修复 烧伤 大鼠 

分 类 号：R459.9[医药卫生—治疗学]