同位素标记结合中性丢失扫描鉴定白蛋白早期糖基化修饰肽段  

Identification of Early Glycated Peptides of Albumin by a Method of Isotopic Labeling Combined with Neutral Loss Scan

作　　者：苏锐[1,2] 王云[1] 刘亚辉[1] 洪杰[1] 张永谦[1] 李玉娟[1] 庆宏[1] 邓玉林[1] 

机构地区：[1]北京理工大学生物医药成分分离与分析实验室,北京100081 [2]中北大学化工与环境学院,山西太原030051

出　　处：《北京理工大学学报》2014年第12期1310-1314,共5页Transactions of Beijing Institute of Technology

基　　金：国家"九七三"计划项目(2012CB910603;2012YQ040140-10)

摘　　要：采用[13 C6]同位素标记的葡萄糖作为标签,在体外建立非酶糖基化反应模型,并采用MALDI-TOF对模型中的糖基化人血清白蛋白(HSA)进行验证.以高分辨率的液质联用四级杆飞行时间质谱(HPLC/Q-TOF MS)为分析平台,经过一级质谱(LC/MS)筛选糖基化肽段.采用target MS/MS模式对筛选出的34条肽段进行分析.运用蛋白质组学数据分析工具pFind软件对肽段的中性丢失扫描及二级质谱(LC/MS/MS)数据进行分析,最终获得16条早期糖基化修饰肽段的序列及修饰位点信息.This project utilized isotopic labeling with [^13C6]glucose as a tag,establishing nonenzymatic glycation reaction model in vitro.Glycated the model human serum albumin（HSA）was verified using MALDI-TOF.The glycated peptides from the digestion HSA were selected by LC/MS on a high resolution HPLC/Quadrupole time of flight mass（HPLC/Q-TOF MS）analysis platform.The 34 screened glycated peptides were analyzed using target MS/MS mode.The pFind software,which is a proteomics data analysis tool,was applied for the automated data analysis of the neutral scan and LC/MS/MS data.Finally,this method allowed to obtain the information of sequences and modified site of 16 early glycated peptides.

关 键 词：同位素标记 中性丢失 糖基化肽段 

分 类 号：Q51[生物学—生物化学]