抑制山女鳟源水霉菌菌丝及游动孢子生长的药物筛选  

Screening of Inhibitory Medicines to Hyphae and Zoospore from Oncorhynchus masou masou saprolegniasis

作　　者：甄珍[1,2] 王荻[2] 范兆廷[1] 刘红柏[2] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]中国水产科学研究院黑龙江水产研究所,哈尔滨150070

出　　处：《中国农学通报》2014年第35期116-120,共5页Chinese Agricultural Science Bulletin

基　　金：公益性行业(农业)科研专项经费"冷水性鱼类病害防治技术研究"(201003055)

摘　　要：研究了药物对山女鳟水霉病病原的抑制及杀灭效果,即中草药抑制水霉菌丝生长和抗水霉制剂抑制孢子的生长。实验选用过氧化氢、甲醛等6种常用抗水霉制剂及地肤子、蛇床子等16种中草药单方和2种复方(T1、T2)分别采用孢子计数法和琼脂平板扩散法对水霉游动孢子及菌丝的体外抑菌效果进行研究。研究结果显示,过氧化氢和甲醛抑孢子的效果优于孔雀石绿,而检科Ⅰ号、亚硝酸钠和过氧乙酸的抑制效果不及孔雀石绿。石榴皮和复方T1能有效抑制菌株SN的菌丝生长;地肤子、肉桂、柠檬、苦参、大蒜、黄精、茴香和菖蒲对菌株几乎无抑菌作用;黄芩、虎杖、金银花、苦楝皮、复方T2、蛇床子、土槿皮和大黄均有较好的抑制作用。实验为合理的筛选有效抑山女鳟水霉菌药物及制定科学有效地抗霉剂使用方法提供理论依据。The paper aims to study the effects of drugs that inhibit and kill pathogens, including Chinese Medicinal herbs that inhibit hyphae growth and anti-Saprolegnia agents that inhibit zoospore. The authors chose 6 commonly used anti-Saprolegnia agents （such as hydrogen peroxide, formaldehyde et al. ） and 16 Chinese medicinal herbs （such as Fructus kochiae Scopariae, Cnidium rnonnieri et al.） and 2 compound prescriptions （T1 and T2）, used the method of spore counted and agar plate to conduct the research. The results show that hydrogen peroxide and formaldehyde inhibit spore better than malachite green, but the inhibitory effect of Jianke I, sodium nitrite and peracetic acid are less than malachite green. The pomegranate rind and compound T1 can effectively inhibit the hypha growth; Fructus kochiae Scopariae, Cinnamomum cassia Presl, Citrus limon, Radix Sophorae flavescentis, Allium sativum, Polygonatum sibiricum, Foeniculum vulgare and Acorus calamus have almost no inhibitory effect on the strain; Scutellaria baicalensis Georgi, Reynoutria japonica Houtt, Lonicera japonica Thunb, Cortex meliae, compound T1, Cnidium monnieri, Pseudolarix kamepferi Gord and Rheum palmatum have better inhibitory effect. The experiment provides theoretical basis for screening the medicines which have the efficacious antibacterial activity on Saprolegnia in Oncorhynchas masou masou and developing scientific and effective utilization method of anti-Saprolegnia agents.

关 键 词：山女鳟 水霉菌 中草药 抗水霉制剂 抑制 

分 类 号：S941.431[农业科学—水产养殖]