不同产地山羊角凝胶电泳指纹图谱及其混伪品鉴别研究  

Studies on SDS-PAGE Fingerprints Chromatogram of Capra hireus from Different Areas and Its Adulterants

作　　者：康馨元[1] 刘睿[2] 李春楠[1] 孙佳明[1] 张辉[1] 

机构地区：[1]长春中医药大学,吉林长春130117 [2]南京中医药大学,江苏南京210046

出　　处：《中国现代中药》2015年第1期20-23,共4页Modern Chinese Medicine

基　　金：角类动物药的标准制定项目--国家药品标准提高暨2015版药典科研任务(2012)

摘　　要：目的:研究建立不同产地山羊角药材的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)的指纹图谱以及与混伪品的鉴别方法。方法:采用SDS-聚丙烯酰胺凝胶电泳法对山羊角及其伪品的蛋白成分进行分析比较。结果:不同产地山羊角电泳图谱相似,有5个共有带峰,可作为山羊角的专属条带。结论:该方法简单易行、灵敏度高,可为山羊角的产地鉴别及与伪品的鉴别提供参考。Objective：To establish the SDS PAGE fingerprints and an identification method for Capra hireus Lcollected from different areas and its adulterantMethods：The SDS PAGE method was adopted to compare the difference of Chireus and its adulterantsResults：It showed a similar electrophoretic patterns for CHireus of different originsFive common peaks were determined as characteristic bandsConclusion：The established method is simple and sensitiveIt can provide the basis for identification of habitats and adulterants

关 键 词：山羊角 十二烷基硫酸钠 聚丙烯酰胺凝胶电泳 指纹图谱 

分 类 号：R512.5[医药卫生—内科学]