DNA倍体分析在良恶性支气管刷片或支气管灌洗液中的诊断价值  

Clinical Value of DNA Ploidy Analysis in the Diagnosis of Benignancy and Malignancy with Bronchial Brushing or Bronchoalveolar Lavage Fluid

作　　者：毛志远[1] 熊梅[2] 任力[1] 李炜[3] 李德昌[1] 岳颖[1] 郑吉春[1] 

机构地区：[1]中国人民解放军空军总医院病理科,北京100142 [2]中国人民解放军空军总医院输血科,北京100142 [3]同济医学院中加肿瘤研究中心

出　　处：《华西医学》2014年第12期2226-2229,共4页West China Medical Journal

摘　　要：目的探讨DNA倍体分析系统在良恶性支气管刷片或支气管灌洗液中的诊断价值。方法 2012年6月-2013年6月96例病理诊断明确的良恶性支气管刷片或支气管灌洗液标本,其中良性49例,恶性47例。支气管刷片由临床医生进行支气管镜检查时刷取2张刷片,支气管灌洗液标本经离心涂片,每例制成2张薄层细胞片。1张常规苏木精-伊红染色,进行常规细胞学检查;另外1张Feulgen染色,利用DNA倍体全自动图像分析仪进行DNA倍体分析。结果常规细胞学对支气管刷片或支气管灌洗液良恶性诊断的特异度、灵敏度、准确率分别为85.7%、78.7%及77.1%,而DNA倍体分析的特异度、灵敏度、准确率分别为100.0%、91.5%及95.8%,两种方法比较差异均有统计学意义(P<0.05)。结论 DNA倍体分析能够提高支气管刷片或支气管灌洗液的阳性检出率,并且在诊断良恶性病变中较常规细胞学更特异、更敏感,具有较高的价值。Objective To investigate the clinical value of DNA ploidy analysis system in the diagnosis of benignancy or malignancy with bronchial brushing or bronchoalveolar lavage fluid. Methods We studied 96 bronchial brush tablets or bronchoalveolar lavage fluid specimens confirmed pathologically between June 2012 and June 2013, in which there were 49 cases of benignancy and 47 malignancies. Bronchial brush pieces were acquired by clinicians when they performed bronchoscopy for the patients. Each bronchoalveolar lavage fluid specimen was made into two slides, of which one was stained by HE method for cytology analysis, and the other was stained with Feulgen method for DNA ploidy analysis through automatic imaging cytometer. Results The specificity, sensitivity and accuracy of the routine cytological investigation were respectively 85.7%, 78.7% and 77.1%, while the three indexes for DNA ploidy analysis were 100.0%, 91.5% and 95.8%, respectively. Conclusion DNA ploidy analysis can improve the bronchial brushing or bronchoalveolar lavage fluid positive rate, and compared with cytological investigation, it is more specific and more sensitive with a high clinical value.

关 键 词：支气管刷片 支气管灌洗液 DNA倍体分析 常规细胞学 

分 类 号：R562.2[医药卫生—呼吸系统]