CD4^+CD25^+Foxp3^+调节性T细胞与IL-33在儿童哮喘发病机制中的作用  

Roles of CD4^+CD25^+Foxp3^+ regulatory T cells and IL-33 in the pathogenesis of asthma in children

作　　者：潘珍珍[1] 李羚[1] 郭赟[1] 贺建[1] 

机构地区：[1]南京医科大学附属无锡市儿童医院呼吸内科,江苏无锡214000

出　　处：《中国当代儿科杂志》2014年第12期1211-1214,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的探讨CD4+CD25+Foxp3+调节性T细胞(Treg)与IL-33在儿童哮喘发病中的作用。方法采用流式细胞仪检测45例哮喘患儿(哮喘组)、50例呼吸道合胞病毒感染喘息患儿(喘息组)及40例健康儿童(对照组)外周血CD4+CD25+Foxp3+Treg细胞百分比,采用ELISA法检测各组外周血血清IFN-γ、IL-4、IL-5及IL-33浓度,进行比较分析。结果哮喘组患儿体内CD4+CD25+Foxp3+Treg水平较喘息组及对照组均降低(P<0.05);哮喘组患儿体内IL-33水平较喘息组及对照组均升高(P<0.05),哮喘组患儿体内CD4+CD25+Foxp3+Treg与IL-33呈负相关(r=-0.156,P<0.01)。结论在哮喘患儿发病机制中,CD4+CD25+Foxp3+Treg与IL-33可能存在相互作用。Objective To study the roles of CD4＋CD25＋Foxp3＋ regulatory T cells （Treg） and IL- 33 in the pathogenesis of asthma in children. Methods Flow cytometry was used to detect peripheral blood CD4＋CD25＋Foxp3＋Treg proportion in CD4＋T lymphocytes in.45 children with asthma, 50 children with wheezing caused by respiratory syncytial virus infection and 40 healthy children. Serum levels of IFN-3, IL-4, IL-5 and IL-33 were measured using ELISA. Results The level of peripheral blood CD4＋CD25＋Foxp3＋Treg in the asthma group was significantly lower than in the wheezing and control groups （P〈0.05）. In contrast, serum levels of IL- 33 in the asthma group was significantly higher than in the wheezing and control groups （P〈0.05）. Peripheral blood CD4＋CD25＋Foxp3＋Treg level was negatively correlated with serum IL-33 level in the asthma group（r=-0.156, P〈0.01）. Conclusions CD4＋CD25＋Foxp3＋Treg may interact with IL-33 in the pathogenesis of childhood asthma.

关 键 词：CD4+CD25+Foxp3+调节性T细胞 IL-33 哮喘 儿童 

分 类 号：R725.6[医药卫生—儿科]