CD4^+CXCR5^+Tfh细胞比例及其调控因素变化对哮喘患儿的影响  

Alterations of CD4^+CXCR5^+Tfh cells and its transcription regulatory factors in children with asthma

作　　者：崔亚杰[1] 陈国洪[1] 王俊玲[1] 马琳[1] 郭晓玲[1] 廖建湘[2] 李成荣[2] 王国兵[2] 

机构地区：[1]郑州市儿童医院呼吸内科,河南郑州450003 [2]深圳市儿童医院儿科研究所,广东深圳518026

出　　处：《中国当代儿科杂志》2014年第12期1215-1219,共5页Chinese Journal of Contemporary Pediatrics

基　　金：郑州市儿童医院重点专科基金

摘　　要：目的探讨哮喘患儿外周血滤泡样辅助性T细胞(CD4+CXCR5+Tfh细胞)比例及其转录调控因子BCL-6、BLIMP-1的表达变化。方法依据病情将64例哮喘患儿分为哮喘急性期组(n=36)和哮喘缓解期组(n=28),另选取同期在我院行健康体检儿童25例为对照组。流式细胞术检测各组外周血CD4+CXCR5+Tfh细胞数量;实时荧光定量PCR仪检测各组转录调控因子BCL-6、BLIMP-1m RNA表达;双抗夹心ELISA法检测血浆总Ig E、IL-2、IL-6、IL-21浓度水平。结果哮喘急性期组患儿CD4+CXCR5+Tfh细胞比例明显高于对照组及哮喘缓解期组(P<0.05);哮喘急性期组患儿BCL-6m RNA表达水平显著高于对照组及哮喘缓解期组(P<0.05),而BLIMP-1m RNA表达水平明显低于对照组及哮喘缓解期组(P<0.05);哮喘急性期组患儿血浆IL-6水平显著高于对照组(P<0.05),血浆IL-21和总Ig E水平显著高于对照组和哮喘缓解期组(P<0.05),血浆IL-2水平明显低于对照组和哮喘缓解期组(P<0.05);血浆IL-21和IL-6水平与CD4+CXCR5+Tfh细胞数量呈正相关(分别r=0.76、0.46,均P<0.05),与IL-2水平呈负相关(r=-0.68,P<0.05)。结论外周血CD4+CXCR5+Tfh细胞比例增高可能参与了儿童哮喘的急性发病过程,转录调控因子BCL-6及BLIMP-1m RNA的异常表达,以及局部微环境中总Ig E和细胞因子IL-2、IL-6、IL-21的浓度改变可能与哮喘患儿CD4+CXCR5+Tfh细胞分化异常有关。Objective To study the alterations of follicular T helper cells （CD4＋CXCR5＋Tfh cells, Tfh） on circulating T lymphocytes in children with asthma, and to study the expression of transcription regulatory factors BCL- 6 and BLIMP-1 mRNA. Methods Sixty-four children with asthma and 25 healthy controls were enrolled in this study. On the basis of the disease, the children with asthma were classified into acute phase group （n=36） and remission phase group （n=28）. The flow cytometry was used to detect the proportion of CD4＋CXCR5＋Tfh cells on CD4＋T lymphocytes. Real-time PCR was performed to detect the levels of BCL-6 mRNA and BLIMP-1 mRNA. The double -antibody Sandwich ELISA was used to detect plasma concentrations of total IgE, IL-2, IL-6 and IL-21. Results The proportion of CD4＋CXCR5＋Tfh cells was significantly higher in the acute group than in the control group and the remission group （P〈0.05）. Transcription levels of BCL-6 mRNA were significantly higher, while the inhibitory factors BLIMP-1 mRNA was significantly lower in the acute group than in the remission group and control group （P〈0.05）. The plasma concentration of IL-6 in the acute group increased significantly compared with the control group （P〈0.05）. Plasma concentrations of total IgE and IL-21 increased significantly, in contrast, plasma IL-2 concentration decreased significantly in the acute group, compared with the control group and the remission group （P〈0.05）. Correlation analysis showed that both IL-21 and IL-6 concentrations were positively correlated with the proportion of CD4＋CXCR5＋Tfh cells （r=0.76, r=0.46 respectively; P〈0.05）, while IL-2 level was negatively correlated with the proportion of Tfh cells （r=-0.68, P〈0.05）. Conelusions The abnormal proportion of CD4＋CXCR5＋Tfh cells might be involved in the immunological pathogenesis of acute asthma in children. The increased expression of BCL-6 mRNA and decreased expression of BLIMP-1 mRNA as well as the alterations of plasma total

关 键 词：滤泡样辅助性T细胞 BCL-6 BLIMP-1 白介素 哮喘 儿童 

分 类 号：R725.6[医药卫生—儿科]