双氢青蒿素对C2株蓝氏贾第鞭毛虫Alpha-7.3giardin基因mRNA表达水平的影响  

Effects of dihydroartemisinin on the expression level of Alpha-7.3 giardin mRNA in C2 Giardia lamblia

作　　者：余源[1] 陈阳[1] 葛爽[1] 王洋[1] 李巍伟[1] 赵丽娜[1] 刘阿倩 林志强[1] 高雪[1] 田喜凤[1] 

机构地区：[1]河北联合大学生命科学学院,唐山063000

出　　处：《中国人兽共患病学报》2014年第7期669-672,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.30970313);唐山市科技支持计划项目(No.12140209A-33)联合资助~~

摘　　要：目的观察双氢青蒿素(dihydroartemisinin,DHA)对C2株蓝氏贾第鞭毛虫(Giardia lamblia)Alpha-7.3giardin(α-贾第素)基因mRNA表达水平的影响,探讨其对蓝氏贾第鞭毛虫骨架蛋白的损伤作用。方法用双氢青蒿素浓度为100μg/mL、200μg/mL的改良TYI-S-33培养基分别培养C2株蓝氏贾第鞭毛虫2h、4h、8h、12h后,以不含药物组为对照,实时荧光定量RT-PCR检测药物作用后Alpha-7.3giardin基因mRNA表达水平的变化。结果双氢青蒿素作用虫体后Alpha-7.3giardin基因mRNA表达水平明显低于对照组,二者有显著性差异。结论双氢青蒿素对C2株蓝氏贾第鞭毛虫Alpha-7.3giardin基因mRNA的表达具有明显的抑制作用,抑制效果与药物浓度和作用时间相关,提示双氢青蒿素对蓝氏贾第鞭毛虫骨架蛋白具有损伤作用。Effects of dihydroartemisinin （DHA） on the expression level of Alpha-7 .3 giardin mRNA in C2 Giardia lam-blia was investigated in this study to explore the damage to skeleton protein of C 2 Giardia lamblia .Giardia lamblia was culti-vated respectively for 2 ,4 ,8 ,and 12 hours with modified TYI-S-33 medium containing 100 μg/mL and 200 μg/mL DHA , while the control group performed in the same experimental conditions without DHA .The expressive quantity of Alpha-7 .3 gi-ardin mRNA was determined by using real-time reverse transcription PCR ,and then we found that the expressive quantities of Alpha-7 .3 giardin mRNA with DHA were significantly lower than those in the control group .It’s suggested that dihydroarte-misinin has obvious inhibitory effect on the expression level of Alpha-7 .3 giardin mRNA in C2 Giardia lamblia .The actions of dihydroartemisinin on skeleton protein of C2 Giardia lamblia are effective .

关 键 词：C2株蓝氏贾第鞭毛虫 双氢青蒿素 Alpha-7.3 giardin 实时荧光定量RT—PCR 

分 类 号：R382.2[医药卫生—医学寄生虫学]