姜黄素抑制超抗原TSST-1诱导脾细胞炎症因子的作用  

Effect of curcumin on superantigen TSST-1 induced inflammatory cytokines by splenocytes

作　　者：温晓婷[1] 黄元铭[2] 王智昊[2] 任志鸿[2] 卢珊[2] 

机构地区：[1]山西医科大学公共卫生学院,太原030001 [2]中国疾病预防控制中心传染病所,北京102206

出　　处：《中国人兽共患病学报》2014年第7期727-732,共6页Chinese Journal of Zoonoses

基　　金：传染病预防控制国家重点实验室留学归国人员启动经费(No.2011SKLID301)~~

摘　　要：目的观察姜黄素对毒性休克综合征毒素-1(TSST-1)诱导的小鼠脾细胞炎症因子的影响,为进一步探讨姜黄素对炎症性休克的作用提供依据。方法采用乳酸脱氢酶(LDH)释放法检测不同剂量的TSST-1和姜黄素的细胞毒作用,ELISA和流式细胞术检测相关炎症因子。结果所选剂量TSST-1和姜黄素均未发现有明显细胞毒作用。TSST-1诱导脾细胞产生了大量的IFN-γ和IL-2,产生了少量的TNF-α,未能诱导出IL-1β、IL-6和IL-12。TSST-1诱导的Th1细胞因子产生不完全依赖于IL-12。姜黄素对IFN-γ和TNF-α的抑制作用与剂量有关,在15μmol/L抑制效果最显著(P<0.05),但对IL-2无显著抑制作用(P>0.05)。结论姜黄素能显著降低TSST-1诱导小鼠脾细胞产生的IFN-γ和TNF-α,但对T细胞增殖无明显抑制作用。In the present study ,we aimed to observe the effect of curcumin on TSST-1-induced inflammatory cytokines in splenocytes of mouse and provide evidence for the further study on the effect of curcumin on inflammatory shock .Lactate de-hydrogenase （LDH） release assay was used to determine cytotoxicity of different doses of TSST-1 and curcumin .Inflammatory cytokines were determined by ELISA and flow cytometry .The doses of TSST-1 and curcumin we used in the present study did not cause significant cytotoxicity .TSST-1 induced higher level of IFN-γand IL-2 production but relatively lower level of TNF-α.The production of IL-1β,IL-6 and IL-12 was undetectable .TSST-1 induced Th1 cytokines （IFN-γand IL-2） were not IL-12-dependent which was different from LPS-induced IFN-γ.Curcumin significantly reduced IFN-γand TNF-αproduction at the concentration of 15 umol/L （P〈0 .05） ,but had no effect on IL-2 production （P〉0 .05） .It’s suggested that curcumin could significantly inhibit the production of IFN-γand TNF-αby splenocytes induced by TSST-1 ,but could not affect the prolifera-tion of T cells .

关 键 词：毒性休克综合症毒素-1 姜黄素 炎症性休克 炎症因子 

分 类 号：R378.1[医药卫生—病原生物学]