猪2型链球菌安徽分离株的毒力基因、溶血性及耐药性分析  

Virulence genes,hemolytic and antibiotic resistance in Streptococcus suis serotype 2 isolated from Anhui Province,China

作　　者：焦安心 许大文[1] 赵洪武[1] 程谦[1] 郑浩[1] 马建华[1] 魏建忠[1] 孙裴[1] 李郁[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《中国人兽共患病学报》2014年第12期1181-1186,共6页Chinese Journal of Zoonoses

基　　金：安徽省长三角联合攻关项目(No.1101c0603065);安徽农业大学学科学位点建设项目(No.XKXWD2013006);安徽农业大学大学生创新基金项目(No.2011053);安徽省生猪产业体系(No.2010)联合资助~~

摘　　要：目的了解猪2型链球菌（S.suis 2）安徽分离株的毒力基因分布特征以及溶血性和耐药性。方法对19株S.suis 2安徽分离株,采用PCR方法检测cps2J、mrp、epf、sly4种毒力基因,PA法和micro-ELISA法检测溶血类型和溶血价,K-B法检测对25种抗生素的敏感性。结果 11株毒力基因型为cps2J＋/mrp＋/epf＋/sly＋,占总菌株的57.9%,为毒力优势基因型;19株均呈α或β溶血,溶血价为1∶4~1∶128;对利福平、头孢他啶、氟苯尼考、头孢唑啉的敏感率较高,平均为84.2%,对强力霉素、四环素、杆菌肽和磺胺异恶唑的耐药率较高,平均为82.9%,且对该4种抗生素的多重耐药率为63.2%。结论安徽猪群中流行的S.suis 2毒力基因的分布特征与国内相似,与国外存在一定差异,CPS、MRP、EPF和SLY是重要的毒力因子;S.suis 2的溶血类型与其SLY有关,但sly基因的不完整性并不一定改变S.suis 2的溶血性;S.suis 2安徽分离株的多重耐药性严重,耐药谱与其他地区存在一定差异。The virulence genes, hemolytic and antibiotic resistance characteristics from 19 isolated Streptococcus suis serotype 2 in Anhui Province were investigated in this study. The PCR method was developed for detecting cps2J, mrp, epf and sly gene; the plate method and micro-ELISA were developed for detecting the hemolytic type and hemolytic titer; the K-B method was developed for detecting antibiotic resistance of 25 kinds of antibiotics. Results showed that there were 11 strains with the dominant virulence genotype of cps2J＋/rnrp＋/epf＋/sly＋ accounting for 57.9%. All strains were a-hemolysis or β-hemolysis, with hemolytic titer of 1：4 to 1 ： 128. Strains sensitive to rifapentine, ceftazidime, florfenicol and cefazolin was existed in high frequency with the sensitive rates for 84.2%, and the resistance to doxycycline, tetracycline, bacitracin and sul- famethoxazole was existed in high frequency with the resistance rates for 82.9%. The multi-antibiogram typing was doxycyc- line, tetracycline, bacitracin and sulfamethoxazole account for 63. 20%. In conclusion, the distribution of virulence genes of S. suis 2 Anhui isolates was similar with that of domestic reported strains, and there was some differences with that of over- seas reported strains. CPS, MRP, EPF and SLY are important virulence factors and the incompleteness of sly gene had no effect to its haemolyticus. The multi-antibiotic resistance of S. suis 2 Anhui isolates was serious. There is difference for anti-biogram typing between S. suis 2 Anhui isolates and those in other areas.

关 键 词：猪2型链球菌 安徽分离株 毒力基因 溶血性 耐药性 

分 类 号：R378.1[医药卫生—病原生物学]