DNA条形码技术鉴定我国部分白蛉蛉种  

Species identification of partial sandflies from China with DNA barcoding

作　　者：周正斌[1] 张仪[1] 吕山[1] 施文琦[1] 金长发[1] 朱淮民[2] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾,血吸虫病和丝虫病合作中心,上海200025 [2]第二军医大学病原生物教研室,上海200433

出　　处：《中国人兽共患病学报》2014年第12期1209-1213,共5页Chinese Journal of Zoonoses

基　　金：国家十二五重大专项(No.2012ZX10004219);国家科技重大专项(No.2012ZX10004220)联合资助~~

摘　　要：目的探讨DNA条形码技术在白蛉物种鉴定中的可行性。方法通过研究白蛉亚科3个属9个物种的线粒体细胞色素C氧化酶亚基I基因序列,以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法构建系统发育树。结果种内平均遗传距离(0.8%)远远小于种间平均遗传距离(11.2%),同种个体聚为高支持度的单一分支。结论基于线粒体COI基因的DNA条形码可以将不同蛉种很好的区分开来,可以作为一种有效的工具在白蛉物种鉴定中应用。To explore the feasibility of DNA barcoding in the identification of sandflies, the mitochondrial cytochrome c oxidase subunit I （COD genes of 9 dominant species in China from three genera in the Phlebotominae were studied. The in- traspecies and interspecies genetic distances were calculated using the Kimura-2-parameter model, and the phylogenetic trees were reconstructed by neighbor-joining （NJ） method. Results showed that all of sandflies species were successfully distinguish- ed by the phylogenetic trees. The average intraspecific genetic distance （0.8%） was much less than the average interspecific ge- netic distance （11.2%）. The study based on COI sequences indicates that the sandflies from China could be well distinguished with the commonly used COI barcodes, which are potentially well utilized in sandflies species identification.

关 键 词：白蛉 COI DNA条形码技术 

分 类 号：R384.1[医药卫生—医学寄生虫学]