芜菁挥发油对高脂高糖小鼠降血糖的作用机制  

Hypoglycemic mechanism of volatile oil of plateau plant Brassica rapa L.in hyperlipidemic and hyperglycemic mice

作　　者：陈湘宏[1] 刘燕[1] 翁裕馨[1] 康文娟[1] 杨仕兵[1] 

机构地区：[1]青海大学医学院药学系,青海西宁810001

出　　处：《山东大学学报（医学版）》2014年第12期20-23,共4页Journal of Shandong University:Health Sciences

基　　金：教育部春晖计划(Z2011008)

摘　　要：目的探讨芜菁挥发油成分对高脂高糖小鼠动物模型的降血糖作用机制。方法采用超临界二氧化碳萃取仪提取芜菁挥发油成分。高糖高脂饲料喂养小鼠4周后,连续3 d腹腔注射链脲佐菌素(STZ)100 mg/kg,制备小鼠2型糖尿病模型,血糖测定仪尾静脉采血,测空腹血糖,小鼠血糖大于11.0 mmol/L即为造模成功。将小鼠随机分为空白对照组、模型组、芜菁挥发油高剂量[40 g/(kg·d)]组、芜菁挥发油低剂量[20 g/(kg·d)]组、阳性药物(盐酸二甲双胍)组,每日灌胃1次,连续14 d,分别于造模前,造模后,用药后3、7及14 d剪尾取血,检测血糖。小鼠摘眼球取血,血清测定糖化血红蛋白(Hb A1c)、甘油三酯(TG)、总胆固醇(TC)、血清胰岛素(INS)水平;剖取胸腺、脾脏,计算脏器指数。结果高、低剂量的芜菁挥发油均可降低2型糖尿病模型小鼠的血糖水平(P<0.01)及Hb A1c水平(P<0.05),升高INS水平(P<0.05),显著降低TG及TC水平(P<0.01),保护胸腺,增强机体的免疫能力。结论芜菁挥发油成分对链脲佐菌素联合高脂高糖小鼠糖尿病模型有较明显的降低血糖及血脂的作用,其机制可能是通过干预Hb A1c的形成、提高INS水平来降低高脂高糖小鼠血糖。Objective To explore the hypoglycemic mechanism of plateau plant Brassica rapa L. （turnip） volatile oil in an animal model of diabetic mice. Methods The volatile oil was extracted using supercritical carbon dioxide extrac- tion method. The diabetic mice model was built by feeding high-fat and high-carbohydrate diet for 4 weeks then intrap- eritoneal injection of streptozotocin （STZ） 100 mg/kg for 3 days. The mice with fasting blood glucose in tail vein being greater than 11.0 mmol/L were defined as successful experimental models. Fifty mice were randomly divided into con- trol group, model group, high dose of turnip volatile oil group, low dose of turnip volatile oil group, and positive con- trol （metformin hydrochloride） group, 10 in each group. The mice were perfused for 14 days at one time per day. The blood was collected from tail vein and the blood glucose level was measured before and after building the models, and at day 3, 7, 14 after medication. The blood samples were taken from the mice, s eyeballs and the glycosylated hemoglobin （HbAlc）, triglyceride （TG）, total cholesterol （TC）, and serum insulin （INS） levels were measured. The fresh spleen and thymus gland were cut and weighed to compute organ index. Results Both high dose and low dose of tur- nip volatile oil had significant influences on the type 2 diabetic model mice, including reduced blood glucose （P 〈 0.01 ） and HbAlc levels （P 〈0.05）, increased INS level （P 〈0.05）, and decreased TG and TC levels （P 〈0.01 ）. The turnip volatile oil could protect the thymus in diabetic mice, and enhance the immune ability. Conclusion Turnip volatile oil can reduce hypexglycemia significantly in diabetic model induced by STZ, which may due to the intervention of the biosynthesis of HbAlc and increase of INS level.

关 键 词：芜菁 糖化血红蛋白 血清胰岛素 总胆固醇 甘油三酯 

分 类 号：R285.5[医药卫生—中药学]