ALA-PDT对皮肤鳞状细胞癌A431细胞Caspase-3及Caspase-7表达的影响  

Effects of ALA-PDT on Caspase-3 and Caspase-7 expressions in cutaneous squamous cell carcinoma A431 cells

作　　者：刘园园[1] 王逸飞[2] 张春敏[1] 刘瑶[1] 魏国[1] 张春红[1] 刘瑛[1] 

机构地区：[1]山东大学第二医院皮肤科,山东济南250033 [2]山东大学医学院,山东济南250012

出　　处：《山东大学学报（医学版）》2014年第12期50-53,59,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科学技术发展计划(2009GG10002026)

摘　　要：目的观察盐酸氨酮戊酸散(ALA)-光动力疗法(PDT)对皮肤鳞状细胞癌A431细胞中Caspase-3及Caspase-7表达的影响。方法体外培养A431细胞,应用M TT法检测不同ALA浓度、不同培养时间、不同PDT光照剂量以及单独加药和单独光照对A431细胞增殖与凋亡的影响,并设Hacat作为对照;应用实时荧光定量PCR及Western blotting检测A431细胞及Hacat细胞中Caspase-3及Caspase-7 mRNA和蛋白的表达水平。结果A431细胞中Caspase-3及Caspase-7 mRNA和蛋白的表达量低于Hacat细胞,其表达强弱差异有统计学意义(P<0.05)。ALA-PDT对A431细胞的增殖具有一定的抑制作用,并引起Caspase-3及Caspase-7 mRNA和蛋白的表达量升高(P<0.01)。当光照剂量为80 J/cm2时,Caspase-3、Caspase-7 mRNA及蛋白的表达量均达到峰值。结论ALA-PDT对皮肤鳞状细胞癌A431细胞的增殖抑制可能是通过诱导Caspase-3及Caspase-7的表达,引起细胞的凋亡从而发挥作用。Objective To investigate the effect of aminolevulinic acid hydrochloride （ALA）-photodynamic therapy （PDT） on Caspase-3 and Caspase-7 expressions in cutaneous squamous cell carcinoma A431 cells. Methods A431 cells were cultured in vitro. The proliferation and apoptosis of A431 cells with different drug concentration, different in- cubation time, different light dose, as well as with drug and light alone, were assessed by MTT. Hacat was as control. The mRNA expressions of Caspase-3 and Caspase-7 were detected by Real-time PCR. The protein expressions of Caspase-3 and Caspase-7 in A431 cells and Hacat cells were detected by Western blotting. Results The mRNA and protein expressions of Caspase-3 and Caspase-7 were lower in A431 cells than in the Hacat cells with statistically signifi- cant difference （ P 〈 0.05 ）. ALA-PDT inhibited the proliferation of A431 cells in a certain extent, and increased the rnRNA and protein expressions of Caspase-3 and Caspase-7 （P 〈0.01 ）. The inhibition on A431 cells could reach the maximum when the light dose was 80 J/cm^2 with the same incubation time and concentration of ALA. Conclusion ALA-PDT can induce the expressions of Caspase-3 and Caspase-7 directly or indirectly, which induce the apoptosis of cutaneous squamous cell carcinoma A431 cells.

关 键 词：光动力疗法 皮肤鳞状细胞癌A431细胞 CASPASE-3 CASPASE-7 

分 类 号：R75[医药卫生—皮肤病学与性病学]