弓形虫感染大鼠坐骨神经传导速度的动态变化  

Dynamic Changes of Sciatic Nerve Conduction Velocity in Rats Infected with Toxoplasma gondii

作　　者：周永华[1] 杨俊峰[2] 许永良[1] 殷钰涵 张英[1] 顾晓琳[2] 杨静[1] 

机构地区：[1]江苏省寄生虫病防治研究所卫生部寄生虫病预防与控制技术重点实验室,无锡214064 [2]南京中医药大学附属无锡市中医院,无锡214072

出　　处：《中国寄生虫学与寄生虫病杂志》2014年第6期437-440,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：江苏省卫生科技基金(No.X200739,X2011030)~~

摘　　要：目的观察弓形虫感染大鼠坐骨神经传导速度的动态变化。方法将20只雄性SD大鼠随机均分为感染组和对照组。感染组大鼠经腹腔注射弓形虫速殖子(2×107/ml)2 ml/鼠,对照组注射等量生理盐水。于感染前、感染后2、4、8和12个月时应用Cantata TM肌电图仪检测大鼠下肢感觉神经传导速度(SNCV)和运动神经传导速度(MNCV)。结果弓形虫感染后2个月,感染组和对照组大鼠SNCV和MNCV的差异无统计学意义(P>0.05);感染后4个月,感染组大鼠SNCV和MNCV开始减慢,并随着病程延长而进行性加重,4、8和12个月时SNCV分别为(35.26±3.02)、(33.57±2.27)m/s和(32.38±2.38);MNCV分别为(25.94±3.20)、(22.75±2.31)和(22.03±2.08)m/s。SNCV和MNCV在感染后4、8和12个月时较对照组分别下降(7.47±2.11)%、(8.92±2.64)%和(12.18±1.94)%,(12.57±1.89)%、(13.72±2.65)%和(15.46±2.37)%,与对照组相比差异均有统计学意义(P<0.05)。结论弓形虫感染4个月后大鼠下肢感觉和坐骨神经传导速度进行性减慢。Objective To observe the dynamic changes of sciatic nerve conduction velocity of Toxoplasma gondii-infected rats at different time points. Methods Twenty SD rats were randomly divided into control group and Toxoplasma gondii infection group. Rats in T. gondii infection group were intraperitoneal injected with 4×107 T. gondii tachyzoites, while those in control group were given equivalent normal saline. Motor and sensory nerve conduction velocities（MNCV, SNCV） in sciatic nerve were measured by Medtronic Keypoint4 Workstation electromyography at pre-infection, and 2, 4, 8, 12 months post-infection. Results Within two months after infection, there was no difference in SNCV and MNCV between control group and infection group（P〉0.05）. From 4 months after T. gondii injection, infected rats began to show the slowness of SNCV and MNCV, which progressed with the course of infection. At 4, 8, and 12 months after infection, SNCV and MNCV of infection group were（35.26±3.02）and （25.94±3.20） m/s, （33.57±2.27） and （22.75±2.31） m/s, and （32.38±2.38） and （22.03±2.08） m/s, respectively. Compared with control group, SNCV and MNCV of infection group reduced by （7.47±2.11）% and （12.57±1.89）%, （8.92±2.64）% and （13.72±2.65）%, and（12.18±1.94）% and （15.46±2.37）%, respectively（P〈0.05）. Conclusion From 4 months after infection, Toxoplasma gondii-infected rats show a slowness of motor and sensory nerve conduction velocities in sciatic nerve.

关 键 词：弓形虫 感染 坐骨神经 神经传导速度 

分 类 号：R382.5[医药卫生—医学寄生虫学]