骨髓间充质干细胞与人脐静脉内皮细胞的共培养  

Coculture of human umbilical vein endothelial cells and bone marrow mesenchymal stem cells

作　　者：罗淑平[1] 杜玉婷 白驹 

机构地区：[1]解放军兰州军区兰州总医院重症医学科,甘肃省兰州市730050 [2]成都市亚非牙科,四川省成都市610000

出　　处：《中国组织工程研究》2014年第45期7370-7374,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:将人脐静脉内皮细胞与骨髓间充质干细胞共培养来扩增骨髓间充质干细胞数量,同时促进血管生成,可能为骨组织工程和骨再生提供了一个很有前景的方法。目的:对近年国内外关于人脐静脉内皮细胞与骨髓间充质干细胞共培养的研究及其相关进展进行综述。方法:用计算机检索中国知网(2000年1月至2012年3月)、Web of Knowledge数据库及Pub Med数据库(1980年1月至2012年3月)关于干细胞共培养的文献。中文关键词为"人脐静脉内皮细胞、骨髓间充质干细胞细胞、细胞共培养、组织工程",英文关键词为"human umbilical vein endothelial cells,bone Mesenchymal stem cells,coculture,tissue engineering"。计算机初检得到135篇文献,排除因研究目的与本文无关及内容重复的研究103篇,共保留32篇文献做进一步分析。结果与结论:通过查阅国内外文献,发现内皮细胞与骨髓间充质干细胞共培养的研究,都围绕着模拟体内环境、细胞与细胞之间的相互作用、共培养体系中培养基的选择、细胞比例的控制等对内皮细胞成血管作用和骨髓间充质干细胞骨生成作用的影响。很多研究对骨组织工程及骨再生治疗都具有重大的作用,但共培养中内皮细胞与骨髓间充质干细胞之间的作用机制以及在人体内的具体调控仍需要进一步的研究与分析。BACKGROUND：Coculture of bone marrow mesenchymal stem cel s and human umbilical vein endothelial cel s can improve both osteogenic and angiogenic outcomes and provide a promising strategy for bone tissue engineering and osteanagenesis. OBJECTIVE：To summarize recent researches and related progresses in coculture of human umbilical vein endothelial cel s and bone marrow mesenchymal stem cel s. METHODS：A computer-based online search of CNKI database from January 2000 to March 2012, PubMed database and Web of Knowledge database from January 1980 to March 2012, was performed with the keywords of“human umbilical vein endothelial cel s, bone mesenchymal stem cel s, coculture, tissue engineering”both in Chinese and English. A total of 135 articles were screened out, 103 of them were excluded due to unrelated study objective and repeated contents, and final y 32 articles were involved in further analysis. RESULTS AND CONCLUSION：At present, studies on coculture of bone marrow mesenchymal stem cel s and human umbilical vein endothelial cel s mainly focus on mimicking in vivo environments, the interactions between cel s, and the influence of different cel ratios and culture media. Most of these researches play important roles in bone tissue engineering and bone regeneration therapy, but the mechanism of action and concrete regulation in 〈br〉 vivo between bone marrow mesenchymal stem cel s and human umbilical vein endothelial cel s stil need further research and analysis.

关 键 词：干细胞 移植 人脐静脉内皮细胞 骨髓间充质干细胞 共培养 成血管作用 成骨作用 

分 类 号：R318[医药卫生—生物医学工程]