大麦游离小孢子的活力与耐盐性  

Viability and Salt Tolerance of Isolated Microspores from Barley(Hordeum vulgare)

作　　者：刘成洪[1,2] 何婷[1,2] 王亦菲[1,2] 郭桂梅[1,2] 高润红[1,2] 徐红卫[1,2] 陈志伟[1,2] 杨沙沙[1,2,3] 黄亦辰[1,2,3] 陆瑞菊[1,2] 黄剑华[1,2] 

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《植物生理学报》2014年第12期1769-1772,共4页Plant Physiology Journal

基　　金：大麦青稞产业技术体系(CARS-05);上海市农业科学院学科建设专项(LY11)

摘　　要：取田间种植的大麦品种‘花30’幼穗游离小孢子,采用荧光素二醋酸酯(FDA)染色法测定其在不同Na Cl浓度胁迫下的活力,用Image-Pro Plus软件对显微系统拍摄的照片进行分析计数,研究了大麦离体小孢子对盐胁迫的耐受性。结果表明:利用FDA染色结合软件显微拍照系统分析可以快速、准确地测定和分析游离小孢子的活力和大小。对本年度大田种植的大麦‘花30’材料,按照成熟方法游离小孢子,获得40%具有活力的小孢子,游离小孢子在含1%Na Cl提取液中至少2 h内活力保持稳定。The young spikes of barley （Hordeum vulgare） cultivar ＇Hua-30＇ grown in field were used to isolate microspores, and cell viability of isolated microspores was measured under salt stress at different NaC1 concen- trations by the fluorescein diacetate （FDA） staining method. The salt tolerance of isolated microspores was evaluated based on the viability analysis by the software of Image-Pro Plus to capture the microscope pictures and count the cells. The results showed that it was convenient to analyze the microspore viability and size by cell staining with FDA and cell identification with the software. About 40% viable microspores could be achieved from barley cv. ＇Hua-30＇ grown in field by established isolation procedures, and the isolated micro- spores were viable in the extraction buffer containing 1% NaC1 for at least 2 h after isolation.

关 键 词：大麦 游离小孢子 细胞活力 盐胁迫 

分 类 号：S512.3[农业科学—作物学]