大麦单倍体的离体培养与快速繁殖  

In Vitro Culture and Rapid Propagation of Barley Haploid

作　　者：何婷[1,2] 陆瑞菊[1,2] 高润红[1,2] 郭桂梅[1,2] 黄亦辰[1,2,3] 杨沙沙[1,2,3] 陈志伟[1,2] 徐红卫[1,2] 李颖波[1,2] 刘成洪[1,2] 黄剑华[1,2] 

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《植物生理学报》2014年第12期1780-1784,共5页Plant Physiology Journal

基　　金：上海市科委基础研究重点项目(12JC1407800);上海市种业发展项目(沪农科种字2011第1号);国家大麦青稞产业技术体系(CARS-05)

摘　　要：以大麦品种‘花30’小孢子培养来源单倍体植株作为起始材料,分别对其丛生芽诱导、扩繁、生根以及再生植株水培等技术进行了研究。结果表明,添加0.5 mg·L^-1噻苯隆（TDZ）的1/2MS培养基适宜丛生芽诱导;添加1.0 mg·L^-1TDZ的1/2MS培养基适宜丛生芽增殖;分别添加400 mg·L^-1水解酪蛋白（CH）、20 mg·L^-1亚精胺（Spd）和1.0 mg·L^-1多效唑（MET）可以促进丛生芽的健壮;添加0.05 mg·L^-1α-萘乙酸（NAA）和1.0 mg·L^-1MET的1/2MS培养基适宜生根。水培7-10 d后,90%以上的再生植株正常生长。In order to establish a stable barley haploid population, the haploid plants derived from microspore culture of barley （Hordeum vulgate） cultivar ＇ttua-30＇ were used as materials, and the induction and rapid prop- agation of multiple buds, rooting and hydroponics of regenerated plantlets were studied. The results showed that the supplement of 0.5 mg·L^-1 thidiazuron （TDZ） in 1/2MS basal medium was suitable for multiple buds induction; the supplement of 1.0 mg·L^-1 TDZ in 1/2MS basal medium was suitable for bud rapid propagation and the addition of 400 mg·L^-1 casein hydrolysate （CH）, 20 mg·L^-1 spermidine （Spd） and 1.0 mg·L^-1 multieffect triazole （MET） improved the state of buds growth. The supplement of 0.05 mg·L^-1 a-naphthaleneacetic acid （NAA） and 1.0 mg·L^-1 MET in 1/2MS basal medium were suitable for rooting of regenerated plantlets, and more than 90% plantlets survived after an acclimation of 7-10 days by hydroponics.

关 键 词：大麦 单倍体 丛生芽 快速繁殖 

分 类 号：S512.3[农业科学—作物学]