双波长RP-HPLC同时测定龙砂颗粒中7种成分  

Simultaneous determination of seven constituents in Longsha Granules by dual-wavelength RP-HPLC

作　　者：瞿燕[1] 李春雪[1] 曾锐[2] 任桂友[2] 张晨 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]西南民族大学民族医药研究院,四川成都610041

出　　处：《中草药》2015年第2期221-225,共5页Chinese Traditional and Herbal Drugs

基　　金：国家"十二五"科技支撑计划项目(2012BA127B07);四川省科技厅科技支撑项目(2011SZ0071)

摘　　要：目的建立同时测定龙砂颗粒中黄柏碱、芦丁、黄芩苷、黄芩素、盐酸小檗碱、槲皮素、苍术素7种成分的RP-HPLC方法。方法采用全时段双波长RP-HPLC,Kromasil C18色谱柱（250 mm×4.6 mm,5μm）;流动相为乙腈-0.02%磷酸水溶液,梯度洗脱;体积流量1.0 m L/min;柱温30℃;检测波长为205 nm（黄柏碱、芦丁、黄芩苷、黄芩素、槲皮素）和340nm（盐酸小檗碱、苍术素）。结果黄柏碱、芦丁、黄芩苷、黄芩素、盐酸小檗碱、槲皮素、苍术素分别在0.011-0.168 mg/m L（r=0.999 9）,0.010-0.160 mg/m L（r=0.999 9）,0.053-0.842 mg/m L（r=0.999 9）,0.014-0.228 mg/m L（r=0.999 8）,0.016-0.266 mg/m L（r=0.999 8）,0.009-0.144 mg/m L（r=0.999 9）,0.002-0.024 mg/m L（r=0.999 7）线性关系良好,平均加样回收率分别为99.83%、100.09%、99.98%、99.54%、100.14%、99.67%、100.06%,RSD分别为1.9%、2.6%、3.7%、2.4%、2.1%、1.8%、3.2%。结论该方法简便、准确、重复性好,可用于龙砂颗粒的质量控制。Objective To establish the RP-HPLC method for the simultaneous determination of phellodendrine,rutin,baicalin,baicalein,berberine hydrochloride,quercetin,and atractylodin in Longsha Granules.Methods The seven components were measured on a C18 column（250 mm × 4.6 mm,5 μm） of Kromasil with a gradient elution of acetonitrile-0.02% phosphoric acidsolution at the wavelengths of 205 nm（phellodendrine,rutin,baicalin,baicalein,and quercetin） and 340 nm（berberine hydrochloride and atractylodin）,a flow rate of 1.0 m L/min,and the column temperature of 30 ℃.Results Phellodendrine,rutin,baicalin,baicalein,berberine hydrochloride,quercetin,and atractylodin showed a good correlation in the ranges of 0.011—0.168 mg/m L（r = 0.999 9）,0.010—0.160 mg/m L（r = 0.999 9）,0.053—0.842 mg/m L（r = 0.999 9）,0.014—0.228 mg/m L（r = 0.999 8）,0.016—0.266 mg/m L（r = 0.999 8）,0.009—0.144 mg/m L（r = 0.999 9）,and 0.002—0.024 mg/m L（r = 0.999 7）.The average recovery rates were 99.83%,100.09%,99.98%,99.54%,100.14%,99.67%,and 100.06%.The RSD were 1.9%,2.6%,3.7%,2.4%,2.1%,1.8%,and 3.2%.Conclusion The method is simple,accurate,and excellent repetition,and can be used to control the quality of Longsha Granules.

关 键 词：RP-HPLC 龙砂颗粒 黄柏碱 芦丁 黄芩苷 黄芩素 盐酸小檗碱 槲皮素 苍术素 

分 类 号：R286.02[医药卫生—中药学]