HPLC法测定金沙藤中绿原酸、咖啡酸和p-香豆酰葡萄糖  

Simultaneous determination of chlorogenic acid,caffeic acid,and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba by HPLC

作　　者：王采奕 胡筱希 闵建国[1] 邹准[1] 周艳林[1] 邹节明[1] 

机构地区：[1]桂林三金药业股份有限公司,广西桂林541004 [2]河北师范大学化学与材料科学学院,河北石家庄050024 [3]南京农业大学理学院,江苏南京210095

出　　处：《中草药》2015年第2期273-275,共3页Chinese Traditional and Herbal Drugs

基　　金：国家科技重大专项(2011ZX09201-201-17);西南民族药协同创新中心资助课题(CICSEM 2013-A3);桂工信投资项目([2013]258)

摘　　要：目的建立一种同时测定金沙藤中绿原酸、咖啡酸和p-香豆酰葡萄糖的方法。方法采用HPLC法,色谱柱为Phenomenex Cl8（250 mm×4.6 mm,5μm）,甲醇-乙腈-1%冰醋酸（5∶5∶90）为流动相,检测波长为325 nm,柱温35℃。结果绿原酸、咖啡酸和p-香豆酰葡萄糖分别在0.368-2.760、0.064-0.480、0.102-0.765μg内呈良好的线性关系,平均加样回收率分别为101.48%、99.56%、101.73%,RSD分别为1.44%、0.92%、1.62%。结论该方法操作简便、准确、重复性好,可用于金沙藤及其提取物的质量控制。Objective To establish a method for the determination of chlorogenic acid,caffeic acid,and 6-O-p-coumaroyl-Dglucopyranose in Lygodii Herba.Methods The determination was developed on Phenomenex C18 column（250 mm × 4.6 mm,5 μm）,methanol-acetonitrile-1% glacial acetic acid（5︰5︰90） was used as mobile phase,the detective wavelength was 325 nm,and the column temperature was 35 ℃.Results The linear ranges of chlorogenic acid,caffeic acid,and 6-O-p-coumaroyl-D-glucopyranose were 0.368—2.760,0.064—0.480,and 0.102—0.765 μg,respectively.The average recoveries were 101.48%（RSD = 1.44%）,99.56%（RSD = 0.92%）,and 101.73%（RSD = 1.62%）,respectively.Conclusion The method is simple,accurate,and highly reproducible,which could be used for the quality control of Lygodii Herba.

关 键 词：金沙藤 p-香豆酰葡萄糖 绿原酸 咖啡酸 HPLC 

分 类 号：R286.022[医药卫生—中药学]