大鼠骨骼肌损伤后不同时间的病理学变化  

Time-dependent changes of pathology after skeletal muscle injury in rats

作　　者：于天水[1,2,3] 官大威[3] 刘力[4] 赵锐[3] 张海东[1,2] 百茹峰[1,2] 郭兆明[1,2] 张艳[2] 张振华[2] 吴镝[2] 

机构地区：[1]司法文明协同创新中心,北京100192 [2]中国政法大学证据科学教育部重点实验室,北京100192 [3]中国医科大学法医学院,辽宁沈阳110001 [4]北京市公安局法医检验鉴定中心,北京100085

出　　处：《中国法医学杂志》2014年第5期431-433,436,I0001,共5页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金青年科学基金项目(81202383);教育部高等学校博士学科点专项科研基金项目(20122104110025);中国政法大学青年教师学术创新团队资助项目

摘　　要：目的观测大鼠-骨骼肌损伤后不同时间点的病理学变化,为损伤时间推断的研究和应用提供依据和参考。方法 SD大鼠40只随机分为实验组(35只)和对照组(5只),实验组用500g重的打击器打击大鼠右下肢,建立大鼠骨骼肌机械性损伤动物模型,并随机均分为伤后6h、12h、1d、3d、7d、10d、14d组。应用HE染色、Mallory染色和图像分析法观测大鼠骨骼肌损伤的病理学改变。结果伤后6h,损伤区骨骼肌变性、间质大量出血和炎细胞浸润;12h^1d,骨骼肌坏死,伴大量炎细胞浸润,以PMNs和MNCs为主,数量分别达到高峰;3d起,坏死骨骼肌被逐渐清除,PMNs和MNCs数量逐渐减少,FBCs和新生骨骼肌开始出现,少量胶原纤维形成;7d,FBCs和新生骨骼肌数量继续增多,伴新生血管生成和胶原纤维沉积;10d^14d,FBCs数量和胶原纤维沉积分别达到高峰。结论大鼠骨骼肌损伤区PMNs、MNCs和FBCs数量,以及胶原纤维沉积随损伤经过时间的规律性变化,可为损伤经过时间推断研究提供参考。Objective To observe pathological changes in different post-traumatic intervals after rats skeletal muscle injury and to provide reference for investigating skeletal muscle wound age determination in future. Methods A total of 40 Sprague-Dawley male rats were divided into control and injured groups （6h, 12h, ld, 3d, 7d, 10d and 14d after injury）. We established a standardized animal model of skeletal muscle contusion in rats, which was controllable and reproducible well using a self-designed mechanical weight-drop （500g） device. A single impact was delivered to the site of the fight posterior limb. Pathological changes after rats skeletal muscle injury were investigated by hematoxylin-eosin （HE） staining, Mallory staining and image analysis. Results Degeneration, hemorrhage, and inflammatory infiltration were present in contused skeletal muscle at 6h post-injury; neerotized skeletal musele fibers were infiltrated by a large number of inflammatory eells, especially PMNs and MNCs. The number of PMNs and MNCs infiltrated into injured sites reached respectively a peak at 12h ld post-injury; from 3d post-injury onward, neerotized skeletal muscle fibers were phagoeytosed gradually. And the number of PMNs and MNCs was decreased gradually. FBCs, collagen fibers and regenerating myofibers were present in injured sites; the number of FBCs and regenerating myofibers augmented remarkably at 7d post-injury. Simultaneously, angiogenesis and collagen fibers were also observed; at 10 - 14d post-injury, the number of FBCs and collagen fibers deposition reached respectively a peak. Conclusion The number of PMNs, MNCs, FBCs, and the percentage of collagen fibers deposition in skeletal muscle injured zones was respectively time-dependent changes, which may provide reference for wound age determination in forensic medicine.

关 键 词：法医病理学 损伤时间推断 多形核白细胞 单个核细胞 成纤维样细胞 胶原纤维沉积 

分 类 号：D919[医药卫生—法医学]