异基因脂肪干细胞对皮肤移植免疫调节的影响  

Allogeneic ADSCs effects on skin allograft immune adjustment

作　　者：张永平[1] 阎贺 张伟[1] 杨彪炳[1] 

机构地区：[1]潍坊医学院整形外科,山东潍坊261041

出　　处：《中国现代普通外科进展》2014年第11期846-850,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨异基因ADSCs对大鼠同种异体皮肤移植抗免疫排斥的作用。方法:3-4周Wistar大鼠30只,雌雄不限,体质量150-300g,取新西兰大白兔肾周脂肪行传代培养ADSCs至第四代鉴定、备用;将30只大鼠随机等分三组,A组为自体皮肤移植组,B组为同种异体皮肤移植+PBS局部注射组,C组为同种异体皮肤移植+ADSCs局部注射组;术后3、7、14 d观察皮片成活情况、白细胞计数、巨噬细胞活化率、IL-2、IL-10、IFN-γ表达水平,行统计学分析。结果:A、B、C组大鼠移植皮片坏死时间、血液白细胞计数、巨噬细胞活化率及ELISA检测IL-2、IFN-γ、IL-10含量均表明C组与A、B组差异有统计学意义(P<0.05)。结论:脂肪来源干细胞在体内具有较低的免疫原性,对大鼠同种异体皮片移植有抗免疫排斥的作用。Objective： To evaluate the influence of skin allograft for local inject the allogene ADSCs. Methods： Thirty 3-4weeks wistar rats, no male or female, weight 150-300g,drawing New Zealand white rabbit＇s perinephrit fat, routine subculture ADSCs, from the fourth generation. The thirty rats were randomly divided into three groups, every group has ten rats, A group was auto-skin grafting, B group was skin allograft and local inject PBS, C group was skin atlograft and local inject AD- SCs; Observing the skin graft survival time, white blood cell counting, the macrophage activation rate.the expression of IL-2, IL-10,IFN-γin 3, 7, 14 days after the operation and carrying on statistical analysis, Result： A, B, C group ：The average skin graft survival time, the white blood cell count of the rats ,the macrophage activation rate, ELISA tested the content of IL-2, IFN- y, IL-10, group C com- pared with group A, B with statistical significance. Conclusion： Adipose derived stem cells has low immunogenicity in vivo, and has resistance to immune rejection of the rats＇ skin atlograft.

关 键 词：脂肪来源干细胞 异基因 同种异体皮肤移植 抗免疫排斥 

分 类 号：R392.1[医药卫生—免疫学]