儿童急性髓系白血病中miR-196b表达及预后意义  

The expressions of miR-196b and its prognostic significances in pediatric acute myeloid leukemia

作　　者：徐丽华[1,3] 岑建农[2] 何海龙[1] 沈宏杰[2] 杨乃超[1] 颜青[1] 胡绍燕[1] 

机构地区：[1]苏州大学附属儿童医院血液肿瘤科,江苏苏州215003 [2]苏州大学附属第一医院,江苏省血液病研究所血液科,江苏苏州215006 [3]江苏省连云港市第一人民医院儿科,江苏连云港222002

出　　处：《临床儿科杂志》2014年第12期1145-1149,共5页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.81170513,No.81370627)

摘　　要：目的通过检测miR-196b在初诊急性髓系白血病(AML)患儿中的表达,评估miR-196b在儿童AML中的临床意义。方法从标本库中抽取52例初诊AML患儿骨髓,利用q RT-PCR方法检测miR-196b表达水平(结果以2-ΔΔCt表示),并与同期30例非白血病患儿标本对照。结果单核系组(M4、M5)的miR-196b表达水平高于非单核系组(M1、M2、M3、M6、M7)和对照组,而非单核系组低于对照组,差异均有统计学意义(P<0.01)。miR-196b表达水平在t(15;17)组最低,11q23(MLL)组最高,差异有统计学意义(P<0.01)。使用NCCN2013预后分组标准,预后良好组miR-196b表达低于预后不良组,差异有统计学意义(P<0.01)。首疗程缓解组miR-196b表达明显低于首疗程未缓解组,差异有统计学意义(P<0.05)。WBC≥100×109/L组miR-196b表达水平高于WBC<100×109/L组,差异有统计学意义(P<0.01)。miR-196b表达水平与初诊时血小板计数呈显著正相关(r=0.302,P=0.030)。结论 miR-196b表达水平在初诊AML预后不良组明显增高,高水平的miR-196b与低缓解率和较差的预后相关。miR-196b有望成为AML患儿治疗新靶点。Objective To evaluate the expression of miR-196b in newly diagnosed pediatric acute myeloid leukemia （AML） and its clinical signiifcance. Methods Fifty-two AML children were enrolled in this study and 30 non-leukemia com-pared children were selected as controls. The expressions of miR-196b were detected in bone marrow samples by real-time quan-titative PCR （q-RT-PCR） and the results were expressed in 2-△△Ct. Results miR-196b expressions were signiifcantly higher in M4-5 and lower in non-M4-5 of AML children than those in control （P〈0.01）, with a lowest level in t （15;17） and a highest level in MLL subtypes （P〈0.01）. The miR-196b expressions were signiifcantly different among different prognosis groups （P〈0.01） and the level in the favorable prognostic group was lower than in poor prognosis group. It was also found that miR-196b expres-sion was lower in remission group than that in no-remission group after the ifrst induction remission therapy （P〈0.05）. Mean-while, the expression of miR-196b in the children with WBC≥100×10^9/L were statistically higher than that in the children with WBC〈100×10^9/L （P〈0.01）, and miR-196b level was positively correlated with the platelet counts （r=0.302, P=0.030）. Conclu-sions miR-196b expression is increased in poor prognosis group of AML children, and high expression of miR-196b is related with low response rate and poor prognosis. miR-1966 is expected to become a new target for the treatment of AML.

关 键 词：急性髓系白血病 儿童 

分 类 号：R733.71[医药卫生—肿瘤]