脊髓损伤大鼠肠道细菌移位途径的初步探讨  

Route of bacterial translocation in a rat model after spinal cord injury

作　　者：白春宏[1] 彭朋[2] 刘浩[1] 李双英[3] 

机构地区：[1]武警后勤学院附属医院脊柱一科,天津300162 [2]武警后勤学院军事训练医学教研室,天津300162 [3]武警后勤学院附属医院脑科分院,天津300162

出　　处：《重庆医科大学学报》2014年第11期1608-1612,共5页Journal of Chongqing Medical University

基　　金：国家自然科学青年基金资助项目(编号:81101441);武警后勤学院博士启动基金资助项目(编号:WHB201212);武警医学院附属医院科研种子基金资助项目(编号:FYM201111)

摘　　要：目的:初步探讨脊髓损伤大鼠肠道细菌移位的途径。方法:1绿色荧光蛋白标记大肠杆菌肠道定植。224只Wistar大鼠分为正常组(NG=8),损伤组24 h/48 h(EG1=8/EG2=8)。3肠系膜淋巴结、肝脏细菌培养,门静脉血测内毒素;小肠进行光镜、荧光显微镜及电镜观察;肝脏、肠系膜淋巴结进行光镜观察。结果:1肠黏膜屏障破坏明显,细菌通过肠黏膜上皮细胞和裂隙进行跨壁;2淋巴结损伤明显,菌培养:EG1(100%,8/8),EG2(100%,8/8),菌落数EG1开始显著增多;3肝脏损伤明显,菌培养EG1(87.5%,7/8),EG2(100%,8/8),菌落数在EG1开始增多;4EG1(4.11±1.06)U/ml、EG2(6.94±1.50)U/ml内毒素水平均较正常组(0.70±0.04)U/ml升高(F=69.774,P=0.000)。结论:脊髓损伤后细菌及内毒素增多,肠黏膜通透性增加,细菌跨壁后通过淋巴、血行途径转移;形成内毒素血症,使局部及全身炎症反应扩大,形成恶性循环。Objective：To investigate the route of gut bacterial translocation in a rat model after spinal cord injury （SCI）. Methods：①Escherichia coli labeled with enhance green fluorescent protein was inoculated to track bacterial translocation. ②Twenty-four Wistar rats were divided into normal group（n=8） and injury 24 h group（n=8） and injury 48 h group（n=8）. ③Liver and mesenterie lymph nodes were analyzed with bacterial cuhures;the portal vein was analyzed with endotoxin assays;the small intestines were observed under light microscope,fluorescence microscope and electron microscope;liver and mesenteric lymph nodes were observed under light microscope. Results： ①SCI caused impaired intestinal epithelial barrier function. The escherichia coli translocated through the injured epithelial cell or the leakage to the lamina propria of intestinal villi. ②The lymph nodes were injured. Positive rates：injury 24 h group （100%, 8/8）,injury 48 h group （100%, 8/8）. ③The histologic change of liver was apparent. Positive rates：injury 24 h group （87.5%, 7/8）, injury 48 h group（ 100%, 8/8）. ④Endotoxin levels in injury 24 h group and injury 48 h group were significantly different from that of normal group （F=69.774,P=0.000）. Conclusion：After SCI, the intestinal barrier function is damaged and the bacteria and endotoxin are increased. Due to the increased permeability of intestinal mucosal, the bacteria translocate from the gut to other places through lymphatic and blood routes and form the endotoxemia,which amplifies the local and general inflammation and formulates a vicious circle.

关 键 词：脊髓损伤 细菌移位 途径 

分 类 号：R681.54[医药卫生—骨科学]